Increased plant sterol and stanol levels in brain of Watanabe rabbits fed rapeseed oil derived plant sterol or stanol esters
Increased plant sterol and stanol levels in brain of Watanabe rabbits fed rapeseed oil derived plant sterol or stanol esters
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DOI：
10.1017/s0007114507756532
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发表时间：
2007-11-01
影响因子：
3.6
通讯作者：
Luejtjohann, Dieter
中科院分区：
文献类型：
--
作者：
Fricke, Christiane B.;Schroder, Malene;Luejtjohann, Dieter
含有植物甾醇或甾醇酯的食物有助于降低低密度脂蛋白胆固醇浓度，而低密度脂蛋白胆固醇是心血管疾病的主要危险因素。本研究调查了菜籽油（RSO）衍生植物固醇和甾醇酯的高膳食摄入量是否与纯合子和杂合子渡边兔（家族性高胆固醇血症的动物模型）脑组织中这些成分的水平升高有关。纯合子动物分别饲喂标准饲粮、RSO甾醇或RSO甾酯，杂合子动物在各自饲粮中添加2 g /kg胆固醇，连续饲喂120 d（每组第9天）。采用高灵敏度气相色谱-质谱法测定了脑组织、肝脏和血浆中胆固醇、其前体胆甾醇、植物甾醇和甾醇的浓度。大剂量摄入RSO衍生的植物甾醇和甾醇导致血浆和肝脏中这些成分的水平增加。在大脑中，植物甾醇和甾醇的摄取有限，这表明这些化合物通过血脑屏障并可能保留在渡边兔的脑组织中。植物甾醇酯喂养降低了大脑、肝脏和血浆中的植物甾醇水平。脑内的胆固醇合成，由肝脏固醇（一种局部替代胆固醇合成标志物）表示，似乎不受植物固醇或甾醇酯喂养的影响。我们的结论是，高剂量摄入植物甾醇和甾醇酯在渡边兔导致这些成分的浓度升高，不仅在外围，而且在中枢神经系统。
Foods containing plant sterol or stanol esters can be beneficial in lowering LDL-cholesterol concentration, a major risk factor for CVD. The present study examined whether high dietary intake of rapeseed oil (RSO) derived plant sterol and stanol esters is associated with increased levels of these components in brain tissue of homozygous and heterozygous Watanabe rabbits, an animal model for familial hypercholesterolemia. Homozygous animals received either a standard diet, RSO stanol or RSO sterol ester while heterozygous animals were additionally fed with 2 g cholesterol/kg to the respective diet form for 120 d (n 9 for each group). Concentrations of cholesterol, its precursor lathosterol, plant sterols and stanols in brain and additionally in liver and plasma were determined by highly sensitive GC-MS. High-dose intake of RSO derived plant sterols and stanols resulted in increased levels of these components in plasma and liver. In brain a limited uptake of plant sterols and stanols was proven, indicating that these compounds passed the blood-brain barrier and may be retained in the brain tissue of Watanabe rabbits. Plant stanol ester feeding lowered plant sterol levels in brain, liver, and plasma. Cholesterol synthesis in brain, indicated by lathosterol, a local surrogate cholesterol synthesis marker, does not seem to be affected by plant sterol or stanol ester feeding. We conclude that high dose intake of plant sterol and stanol esters in Watanabe rabbits results in elevated concentrations of these components not only in the periphery but also in the central nervous system.