SENDAI VIRUS RECEPTOR - PROPOSED RECOGNITION STRUCTURE BASED ON BINDING TO PLASTIC-ADSORBED GANGLIOSIDES
SENDAI VIRUS RECEPTOR - PROPOSED RECOGNITION STRUCTURE BASED ON BINDING TO PLASTIC-ADSORBED GANGLIOSIDES
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DOI：
10.1073/pnas.77.4.1947
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发表时间：
1980-01-01
期刊：
影响因子：
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通讯作者：
STRANNEGARD, O
中科院分区：
文献类型：
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作者：
HOLMGREN, J;SVENNERHOLM, L;STRANNEGARD, O
确定了仙台病毒与聚苯乙烯培养皿的结合，该培养皿吸附了各种特定结构的神经节苷。通过水蒸气冷凝法或血液吸附法观察神经节苷结合病毒。两种方法均证实了病毒对GT1a、GQ1b和GP1c三种神经节苷元具有高亲和力的特异性结合，这三种神经节苷元在低聚糖部分具有共同的末端序列。除了末端的n -乙酰神经氨酸外，GDIa和GTIb的末端碳水化合物序列相同，但其亲和力仅为末端有二苯甲酰基连接的神经节苷的1-9%。结构NeuAc.alpha。2 .fwdarw。8 NeuAc.alpha。2 .fwdarw。3 gal.beta。1 .fwdarw。galnac是仙台病毒受体的识别特异性结构，存在于细胞膜神经节苷上，也可能存在于糖蛋白上。与塑料吸收糖脂的结合试验被认为是鉴定受体识别结构的有用工具。
The binding of Sendai virus to polystyrene petri dishes to which various gangliosides of defined structures were adsorbed was determined. The ganglioside-bound virus was visualized either by a water vapor condensation method or by a hemadsorption method. By either assay, specific virus binding of high affinity was demonstrated to the gangliosides GT1a, GQ1b, and GP1c which have a common end sequence in the oligosaccharide moiety. Binding also occurred to the GDIa and GTIb gangliosides, which have the same end carbohydrate sequence except for the terminal N-acetylneuraminic acid, but the affinity was only 1-9% of that of the gangliosides with a terminal disialosyl linkage. The structure NeuAc.alpha.2 .fwdarw. 8 NeuAc.alpha.2 .fwdarw. 3Gal.beta.1 .fwdarw. 3GalNAc is the recognition-specific structure of the receptor for Sendai virus that is present on cell membrane gangliosides and possibly also glycoproteins. Binding tests to plastic-absorbed glycolipids are suggested to be a useful tool for identification of the receptor recognition structure.