Analysis of five differentially expressed gene families in fast elongating cotton fiber
Analysis of five differentially expressed gene families in fast elongating cotton fiber
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DOI：
10.1093/abbs/36.1.51
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发表时间：
2004-01-01
影响因子：
3.7
通讯作者：
Zhu, YX
中科院分区：
文献类型：
--
作者：
Feng, JX;Ji, SJ;Zhu, YX
利用抑制消减杂交方法，我们从快速伸长的棉纤维细胞中分离出五个基因家族，包括富含脯氨酸蛋白（PRP）、阿拉伯半乳聚糖蛋白（AGP）、扩展蛋白、微管蛋白和脂质转移蛋白（LTP）。使用cDNA阵列技术的表达谱分析表明，大多数这些基因家族在棉纤维发育早期（花后0-20天，DPA）高度表达。许多转录本在 10 个 DPA 纤维细胞中积累的量是 0 个 DPA 样本中的 50 倍以上。整个基因家族AGP，连同其他4个基因家族的20个个体成员，都是首次在棉花中报道。细胞壁蛋白质、细胞壁松弛酶的积累。微管和脂质转移蛋白可能直接有助于纤维细胞的伸长和发育。
Using the suppression subtractive hybridization method, we isolated five gene families, including proline-rich proteins (PRPs), arabinogalactan proteins (AGPs), expansins, tubulins and lipid transfer proteins (LTPs), from fast elongating cotton fiber cells. Expression profile analysis using cDNA array technology showed that most of these gene families were highly expressed during early cotton fiber developmental stages (0-20 days post anthesis, DPA). Many transcripts accumulated over 50-fold in 10 DPA fiber cells than in 0 DPA samples. The entire gene family-AGP, together with 20 individual members in other 4 Gene families, are reported in cotton for the first time. Accumulation of cell wall proteins, wall loosening enzymes. microtubules and lipid transfer proteins may contribute directly to the elongation and development of fiber cells.