Single molecules solvated in pores of polyacrylamide gels
Single molecules solvated in pores of polyacrylamide gels
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DOI：
10.1080/10587259608042727
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发表时间：
1996-01-01
影响因子：
--
通讯作者：
Moerner, WE
中科院分区：
文献类型：
--
作者：
Dickson, RM;Norris, DJ;Moerner, WE
用远场显微镜在聚丙烯酰胺凝胶的充满水的孔隙中观察到单个荧光分子和单个单标记蛋白质。凝胶框架大大减少了分子的运动范围，从而允许在水环境中长时间研究单个分子。对于小的荧光团，凝胶在每个方向上限制了大约两个数量级的布朗运动，从而大大提高了分子的可探测性。与干聚合物宿主相比，凝胶主要由水组成，大多数分子留在溶液中，因此使这些凝胶成为利用单分子检测方法研究体外生物系统的理想介质。
Individual fluorescent molecules and individual singly-labeled proteins have been observed in the water-filled pores of poly(acrylamide) gels with far-field microscopy. The molecular range of motion is dramatically reduced by the gel framework, thus allowing single molecules to be studied in an aqueous environment for long periods of time. For the small fluorophores, the gel restricts Brownian motion by approximately two orders of magnitude in each direction, thus greatly enhancing the molecule's detectability. In contrast to dry polymeric hosts, the gel is composed primarily of water and the majority of molecules remain in solution, thus making these gels an ideal medium in which to utilize single molecule detection methods for the study of biological systems in vitro.