MICROBIAL EXTRACTION OF CHITIN AND CHITOSAN FROM PLEUROTUS SPP, ITS CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY
MICROBIAL EXTRACTION OF CHITIN AND CHITOSAN FROM PLEUROTUS SPP, ITS CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY
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侧耳菇中甲壳素和壳聚糖的微生物提取、表征及抗菌活性
DOI：
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
R. Ragunathan
中科院分区：
文献类型：
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作者：
J. Johney;K. Eagappan;R. Ragunathan
目的：从两种侧耳菇中提取甲壳素和壳聚糖，研究其理化性质及其抑菌活性。方法：在MGYP培养基中培养15 d，采用碱法和各种生化法提取甲壳素和壳聚糖，并与市售甲壳素和壳聚糖进行比较。同时进行了FTIR和SEM。采用孔扩散法测定其抑菌活性。结果：培养第12天时菌丝体、甲壳素和壳聚糖产量最高。其中，单叶壳聚糖和壳聚糖的产率最高，分别为201.3 mg/l和65.61 mg/l。傅里叶变换红外光谱分析结果与市售甲壳素和壳聚糖的结果比较。结果表明，从侧耳菇中提取的几丁质和壳聚糖与商品有显著的相似性。并对其表面形貌进行了SEM图像研究。真菌甲壳素和壳聚糖对革兰氏菌、革兰氏菌及多种真菌均有较强的抑菌活性。结论：本研究从杏鲍菇中提取了几丁质和壳聚糖。在发酵12天后，产率达到最高。FTIR和SEM也证实了甲壳素和壳聚糖的存在。对所提取的甲壳素和壳聚糖进行抑菌活性研究，发现所提取的甲壳素和壳聚糖对细菌和真菌均有抑菌活性。我们的研究结果表明，在不久的将来，平菇属在药物、人工骨和食品工业原料的开发中具有生产环保型几丁质和壳聚糖的潜力。
Objective: The aim of the present work was to extract the chitin and chitosan from two species of Pleurotus, its characterization and its antimicrobial activity against bacteria and fungi. Methods: The fungi were grown in MGYP medium for 15 d. The chitin and chitosan were extracted using the alkaline method and various biochemical methods and compared with commercial chitin and chitosan. The FTIR and SEM were also performed. Antimicrobial activity was performed using well diffusion method. Results: The maximum yield of mycelia and the chitin and chitosan were obtained on 12 th day of incubation. P. folrida gave maximum yield (201.3 mg/l of chitin and 65.61 mg/l of chitosan). Fourier Transform Infrared spectroscopy comparing the results obtained with results for commercial chitin and chitosan. Our results showed that chitin and chitosan obtained from Pleurotus spp. had a significant similitude with commercial. SEM image also carried out to study the surface morphology. The fungal chitin and chitosan had more antimicrobial activity against the gram+ve, gram–ve and the various fungus. Conclusion: In the present study the chitin and chitosan were extracted from the Pleurotus spp. ( P. florida and P. eous ). The yield was maximum in P. florida after 12 th day of fermentation. The FTIR and SEM also confirmed the chitin and chitosan. The extracted chitin and chitosan were subjected to antimicrobial activity, and it was found the extracted chitin and chitosan were active against the bacteria and fungi. Our findings suggest that the Pleurotus spp. are the potential candidate to produce eco-friendly chitin and chitosan in the development of drugs, artificial bone and raw material for the food industries in the near future.