Surveillance of Enterococcus spp. reveals distinct species and antimicrobial resistance diversity across a One-Health continuum
Surveillance of Enterococcus spp. reveals distinct species and antimicrobial resistance diversity across a One-Health continuum
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DOI：
10.1038/s41598-020-61002-5
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发表时间：
2020-03-03
影响因子：
4.6
通讯作者：
McAllister, Tim A.
中科院分区：
文献类型：
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作者：
Zaheer, Rahat;Cook, Shaun R.;McAllister, Tim A.
对于肠球菌属的抗菌素耐药性（AMR）的一项健康调查，在两年内收集来自人和肉牛沿着以及屠宰场、施肥田、天然溪流和来自城市和牛饲养场来源的废水的分离物。肠球菌属的种属鉴定揭示了整个连续体的不同关联。在收集的8430株分离株中，屎肠球菌和粪肠球菌是城市废水（90%）和临床人体分离株（99%）中的主要菌种;肠球菌在牛（92%）和饲养场集水池（60%）中占主导地位，而天然溪流中含有环境肠球菌属。E.粪肠球菌（n=366株）和E.屎（n=342株），揭示了菌株的来源聚类，表明不同的适应各自的环境。肠球菌属对四环素类和大环内酯类抗生素的表型耐药分别由泰特（M）和tetB（B）编码。不管来源。大肠牛粪便分离株对β-内酰胺类和喹诺酮类的耐药率分别为3%和8%，而人临床分离株的耐药率分别为76%和70%。临床耐万古霉素E.屎肠菌表现出高比率的多药耐药性，对所有测试的β-内酰胺类、大环内酯类和喹诺酮类都具有耐药性。分离株之间AMR谱的差异反映了One-Health连续体每个部门的抗菌药物使用实践。
For a One-Health investigation of antimicrobial resistance (AMR) in Enterococcus spp., isolates from humans and beef cattle along with abattoirs, manured fields, natural streams, and wastewater from both urban and cattle feedlot sources were collected over two years. Species identification of Enterococcus revealed distinct associations across the continuum. Of the 8430 isolates collected, Enterococcus faecium and Enterococcus faecalis were the main species in urban wastewater (90%) and clinical human isolates (99%); Enterococcus hirae predominated in cattle (92%) and feedlot catch-basins (60%), whereas natural streams harbored environmental Enterococcus spp. Whole-genome sequencing of E. faecalis (n=366 isolates) and E. faecium (n=342 isolates), revealed source clustering of isolates, indicative of distinct adaptation to their respective environments. Phenotypic resistance to tetracyclines and macrolides encoded by tet(M) and erm(B) respectively, was prevalent among Enterococcus spp. regardless of source. For E. faecium from cattle, resistance to beta -lactams and quinolones was observed among 3% and 8% of isolates respectively, compared to 76% and 70% of human clinical isolates. Clinical vancomycin-resistant E. faecium exhibited high rates of multi-drug resistance, with resistance to all beta -lactam, macrolides, and quinolones tested. Differences in the AMR profiles among isolates reflected antimicrobial use practices in each sector of the One-Health continuum.