Genetic isolation by distance among Aedes aegypti populations along the northeastern coast of Mexico
Genetic isolation by distance among Aedes aegypti populations along the northeastern coast of Mexico
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DOI：
10.4269/ajtmh.2000.62.200
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发表时间：
2000-02-01
影响因子：
3.3
通讯作者：
Black, WC
中科院分区：
文献类型：
--
作者：
Gorrochotegui-Escalante, N;Munoz, MD;Black, WC
对来自墨西哥东北沿海7个城市的10个埃及伊蚊标本进行了基因流的群体遗传学分析。从蒙特雷收集了四个样本，以研究当地的基因流动模式。标记包括60个随机扩增的多态DNA(RAPD)基因座，以及线粒体DNA(MtDNA)NADH脱氢酶亚单位(MtDNA)387碱基区变异的单链构象多态分析。检测到7个线粒体单倍型，系统发育分析确定了两个支持良好的分支。对地理距离和由随机扩增多态性DNA标记估计的成对F-ST的回归分析表明，群体因距离而被隔离，在90-250公里范围内的样本之间发生了自由的基因流动。用mtDNA单倍型检测不到距离分离。新拉雷多蚊子具有独特的随机扩增多态性DNA和线粒体DNA单倍型频率，杂合度降低，这表明很少有蚊子建立这个种群。
A population genetic analysis of gene flow was conducted among 10 Aedes aegypti collections from seven cities along the northeastern coast of Mexico. Four collections were made from Monterrey to examine local patterns of gene flow. Markers included 60 random amplified polymorphic DNA (RAPD) loci amplified by the polymerase chain reaction and single strand conformation polymorphism analysis of variation in a 387-basepair region of the NADH dehydrogenase subunit 4 from the mitochondrial DNA (mtDNA). Seven mitochondrial haplotypes were detected and phylogenetic analysis identified two well-supported clades. Regression analysis of geographic distances and pairwise F-ST estimated from RAPD markers indicated that populations are isolated by distance and that free gene flow occurs among collections within 90-250 km. Isolation by distance was not detected using mtDNA haplotypes. The Nuevo Laredo collection had unique RAPD and mtDNA haplotype frequencies and reduced heterozygosity suggesting that few mosquitoes established this population.