Quantifying dental biofilm growth using cross-polarization optical coherence tomography.
Quantifying dental biofilm growth using cross-polarization optical coherence tomography.
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DOI：
10.1111/j.1472-765x.2012.03243.x
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发表时间：
2012-06
影响因子：
2.4
通讯作者：
Jones RS
中科院分区：
文献类型：
--
作者：
Chen R;Rudney J;Aparicio C;Fok A;Jones RS
研究了通过交叉偏振 1310 nm 光学相干断层扫描系统 (CP-OCT) 量化复杂的多物种牙齿生物膜的离体生长。将源自儿科受试者牙菌斑样本的细菌微观世界在包含不同牙科材料圆盘的生物膜反应器系统中孵育 72 小时，并每日进行蔗糖脉冲 (5x)。在这些复杂生物膜的各个生长阶段，通过结晶紫测定法验证了生物膜质量的 CP-OCT 分析。 CP-OCT 能够滤除水合生物膜中水层的背反射信号，并允许直接进行生物膜定量。在生物膜的生长阶段，生物膜的整体深度分辨散射强度与结晶紫测定定量（Spearman's ρ = 0.92）表现出非常强的正相关性。 CP-OCT 能够通过测量生物膜的整体深度分辨散射来量化生物膜的质量。 CP-OCT 能够非破坏性地监测生物膜的生长并阐明这些微观世界在不同牙科材料成分上的生长特征。
Quantifying the ex vivo growth of complex multi-species dental biofilms by cross-polarization 1310-nm optical coherence tomography system (CP-OCT) was investigated. Bacterial microcosms, which were derived from plaque samples of pediatric subjects, were incubated in a biofilm reactor system containing discs of different dental materials for 72 hours with daily sucrose pulsing (5x). CP-OCT analysis of biofilm mass was validated with crystal violet assays at various growth stages of these complex biofilms. CP-OCT was able to filter out the back-reflected signals of water layers in the hydrated biofilm and allowed for direct biofilm quantification. The overall depth resolved scattering intensity of the biofilm showed very strong positive correlation to crystal violet assay quantification (Spearman's ρ = 0.92) during the growth phase of the biofilm. CP-OCT was able to quantify the mass of the biofilm by measuring the overall depth-resolved scattering of the biofilm. CP-OCT has the ability to non-destructively monitor biofilm growth and elucidate the growth characteristics of these microcosms on different dental material compositions.