NEUROGENESIS OF THE PERIPHERAL NERVOUS-SYSTEM IN DROSOPHILA EMBRYOS - DNA-REPLICATION PATTERNS AND CELL LINEAGES
NEUROGENESIS OF THE PERIPHERAL NERVOUS-SYSTEM IN DROSOPHILA EMBRYOS - DNA-REPLICATION PATTERNS AND CELL LINEAGES
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DOI：
10.1016/0896-6273(89)90112-8
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发表时间：
1989-07-01
期刊：
影响因子：
16.2
通讯作者：
JAN, YN
中科院分区：
文献类型：
--
作者：
BODMER, R;CARRETTO, R;JAN, YN
利用胸腺嘧啶类似物5-溴-2 ' -脱氧尿苷（BrdU）免疫细胞化学方法研究了产生PNS的细胞系，以显示DNA复制。果蝇胚胎体段中的PNS前体以空间和时间的模版模式复制其DNA。发育中的感觉器官内的DNA复制序列表明构成感觉器官的细胞，即神经元和相关支持细胞的特定谱系关系。在无毛鳞片复合物或子代突变的胚胎中，大部分或全部PNS缺失，在适当区域没有发现brdu标记的细胞，这表明这些PNS前体要么不形成，要么不能复制。因此，BrdU技术可以确定突变是否影响PNS前体或终末分化。
Cell lineages that give rise to the PNS were studied using the thymidine analog 5-bromo-2’-deoxyuridine (BrdU) to visualized DNA replication immunocytochemically, The precursors of the PNS in the body segments of Drosophila embryos replicate their DNA in a spatially and temporally stereotyped pattern. The sequence of DNA replication within developing sensory organs suggests particular lineage relationships of the cells that constitute a sensory organ, ie, neuron and associated support cells. In embryos that are mutant for the achaete-scute complex or daughter/es, in which most or all of the PNS is missing, no BrdU-labeled cells were found in the appropriate regions, suggesting that these PNS precursors either do not form or fail to replicate. Thus, the BrdU technique allows determination as to whether a mutation affects the PNS precursors or terminal differentiation.