An assessment of chromosomal rearrangements in neopolyploids of Lilium hybrids.
An assessment of chromosomal rearrangements in neopolyploids of Lilium hybrids.
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DOI：
10.1139/g10-018
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发表时间：
2010-05
期刊：
影响因子：
3.1
通讯作者：
Maria Puertas;S. Xie;N. Khan;M. Ramanna;L. Niu;A. Marasek-Ciołakowska;P. Arens;J. M. van Tuyl
中科院分区：
文献类型：
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作者：
Maria Puertas;S. Xie;N. Khan;M. Ramanna;L. Niu;A. Marasek-Ciołakowska;P. Arens;J. M. van Tuyl
利用基因组原位杂交技术，对两种新诱导的百合多倍体（新多倍体）进行了染色体重排检测。其中一个群体是通过异源三倍体长花×东方杂种（LLO）和异源四倍体长花×喇叭杂种（LLTT）杂交获得的，这两个群体都是通过体细胞染色体加倍获得的。另一类异源多倍体群体是利用2个不同种间杂种，即Longiflorum × Asiatic（LA）和东方× Asiatic（OA）的2n配子与亚洲亲本回交获得的。GISH清楚地区分了三个组成基因组（L，T和O）的有丝分裂染色体加倍获得的后代的互补。从该人群中共分析了26名个体，没有染色体重排的证据。然而，在减数分裂加倍的异源多倍体后代的情况下，通过GISH观察到相当高的染色体重排频率。减数分裂多倍体中所谓的染色体重排是同源重组而不是易位的结果。此外，在LA杂种中发生减数分裂重组的证据已被证实与减数分裂染色体上的GISH。因此，有证据表明百合属杂种的新多倍体没有任何明显的染色体重排。
Two types of newly induced polyploids (neopolyploids) of Lilium hybrids were monitored for the occurrence of chromosomal rearrangements through genomic in situ hybridization (GISH) technique. One of the populations was obtained through crossing an allotriploid Longiflorum x Oriental hybrid (LLO) with an allotetraploid Longiflorum x Trumpet hybrid (LLTT), both of which were derived from somatic chromosome doubling. The other type of allopolyploid population was derived from meiotic chromosome doubling in which numerically unreduced (2n) gametes from two different interspecific hybrids, namely, Longiflorum x Asiatic (LA) and Oriental x Asiatic (OA), were used to get backcross progeny with the Asiatic parents. GISH clearly discriminated the three constituent genomes (L, T, and O) in the complements of the progeny obtained from mitotic chromosome doubling. A total of 26 individuals were analyzed from this population and there was no evidence of chromosomal rearrangements. However, in the case of meiotically doubled allopolyploid progeny, considerable frequencies of chromosomal rearrangements were observed through GISH. The so-called chromosomal rearrangements in meiotic polyploids are the result of homoeologous recombination rather than translocations. Furthermore, evidence for the occurrence of meiotic recombination in the LA hybrids has been confirmed with GISH on meiotic chromosomes. Thus, there was evidence that neopolyploids of Lilium hybrids did not possess any noticeable chromosome rearrangements.