Characterisation of scaffold architecture and tendons using optical coherence tomography and polarisation-sensitive optical coherence tomography
Characterisation of scaffold architecture and tendons using optical coherence tomography and polarisation-sensitive optical coherence tomography
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使用光学相干断层扫描和偏振敏感光学相干断层扫描表征支架结构和肌腱
DOI：
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发表时间：
2008
期刊：
影响因子：
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通讯作者：
A. E. Haj
中科院分区：
文献类型：
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作者：
Ying Yang;P. Bagnaninchi;Bin Hu;K. Hampson;A. E. Haj
支架材料在组织工程技术构建功能性组织的过程中起着重要的作用。为了产生高度组织化的组织，支架必须具有特定的内部和外部结构。在这里，利用光学相干断层扫描（OCT）来表征各种支架的结构，特别是已经被制造以支持用于肌腱组织工程的单轴取向胶原束的形成的支架。与此同时，偏振敏感OCT（PSOCT）已经建立，以评估人体肌腱中的胶原纤维组织，并在线和非破坏性地监测工程肌腱结构的生长。还评估了机械刺激对肌腱组织形成和组织的调制的影响。结果表明，传统的OCT能够表征支架结构，并且可以定量和定性地确定孔径、孔隙率或微通道尺寸。PSOCT生成了人类肌腱的双折射图像，并证明低双折射图像与较少的显微结构变化相关，与瘢痕组织或退化组织的存在相关;而组织工程肌腱表现出较低程度的双折射。
Scaffolds play an important role in the generation of functional tissues using tissue-engineering techniques. To generate highly organised tissue, scaffolds must have specific internal and external architectures. Here, optical coherence tomography (OCT) is exploited to characterise the architectures of various scaffolds, in particular scaffolds which have been fabricated to support the formation of uniaxially orientated collagen bundle for use in tendon tissue engineering. In parallel, a polarisation-sensitive OCT (PSOCT) has been built to assess the collagen fibre organisation in human tendon and monitor the growth of engineering tendon constructs online and non-destructively. The impact of mechanical stimuli on the modulation of tendon tissue formation and organisation was also assessed. It is shown that conventional OCT is capable of characterising scaffold architecture and the pore size, porosity or microchannel dimension can be determined quantitatively and qualitatively. PSOCT generated birefringence images of human tendon and demonstrated that low birefringence images, associated with fewer microstructural variations, correlated to the presence of scar tissue or degenerated tissue; whereas the tissue-engineered tendon exhibited lower degree of birefringence.