High-Resolution 3D DNA FISH Using Plasmid Probes and Computational Correction of Optical Aberrations to Study Chromatin Structure at the Sub-megabase Scale
High-Resolution 3D DNA FISH Using Plasmid Probes and Computational Correction of Optical Aberrations to Study Chromatin Structure at the Sub-megabase Scale
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DOI：
10.1007/978-1-4939-2253-6_3
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发表时间：
2015-01-01
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影响因子：
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通讯作者：
Heard, Edith
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文献类型：
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作者：
Giorgetti, Luca;Piolot, Tristan;Heard, Edith
表征染色体的三维组织是分子生物学中的基本目标，并且对于理解基因表达如何由远端调控序列（例如增强子）调控至关重要。染色体构象捕获（3C）技术最近揭示了调控元件之间的相互作用似乎发生在拓扑关联结构域（TADs）的上下文中，每个TADs跨越几百个酶，其中染色质纤维优先相互作用。然而，基于3C的数据表示染色质纤维在数百万个细胞上的平均相互作用概率。为了了解每个染色体内染色质构象的变化，需要采用单细胞技术，如3D DNA FISH。然而，由于TAD的尺寸较小（通常
Characterizing the three-dimensional organization of chromosomes is a fundamental goal in molecular biology and will be critical to understand how gene expression is regulated by distal regulatory sequences such as enhancers. Chromosome conformation capture (3C) techniques have recently revealed that the interactions between regulatory elements appear to occur in the context of topologically associating domains (TADs), each spanning few hundreds kilobases, within which the chromatin fiber preferentially interacts. However, 3C-based data represent average interaction probabilities of the chromatin fiber over millions of cells. To understand how variable chromatin conformation is within each TAD, one needs to employ single-cell techniques such as 3D DNA FISH. Given the small size of TADs however (typically