Comparative Analysis of REGγ Expression in Mouse and Human Tissues
Comparative Analysis of REGγ Expression in Mouse and Human Tissues
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DOI：
10.1093/jmcb/mjq009
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发表时间：
2010-08-01
影响因子：
5.5
通讯作者：
Li, Xiaotao
中科院分区：
文献类型：
--
作者：
Yu, Guowu;Zhao, Yanyan;Li, Xiaotao
据报道，REG γ蛋白酶体是破坏蛋白质的捷径。然而，REG γ的生理功能在很大程度上是未知的。为了了解与不同组织或细胞类型相关的REG γ的潜在生物学作用，我们对小鼠20种不同组织中的REG γ基因表达进行了系统分析，随后验证了小鼠组织中的蛋白质表达，并对选定的人体组织中的REG γ基因表达谱进行了生物信息学分析。来自野生型和REG γ敲除小鼠的不同组织中的REG γ分布的比较分析表明，REG γ存在于许多组织中，并且在某些细胞类型中特异性表达。REG γ的最高表达是在睾丸中，并且REG γ的独特表达特征在包括视网膜神经节细胞和浦肯野细胞的神经元子集中被揭示。它也在生殖器官和胃肠器官中表达。这些表达模式表明REG γ在神经系统、生殖系统和具有增殖能力的细胞中具有潜在的重要功能。与其在生殖组织中表达的重要性一致，REG γ缺乏导致窝仔数呈剂量依赖性减少。
The REG gamma proteasome has been reported as a shortcut to destruction of proteins. However, the physiological functions of REG gamma are largely unknown. To understand the potential biological roles of REG gamma associated with different tissues or cell types, we performed systematic analysis of REG gamma gene expression in 20 different tissues from mice, followed by validation of protein expression in mouse tissues and bioinformatic analysis of REG gamma gene expression profiles in selected human tissues. Comparative analysis of REG gamma distribution in different tissues from wild-type and REG gamma knockout mice indicates that REG gamma is present in many tissues and is specifically expressed in some cell types. The highest expression of REG gamma is in the testis and unique expression features of REG gamma are revealed in a subset of neurons, including retinal ganglion cells and Purkinje cells. It is also expressed in reproductive and gastro-intestinal organs. These expressed patterns suggest potentially important functions for REG gamma in the nervous system, reproductive system and in cells with proliferative capacity. Consistent with the importance of its expression in reproductive tissue, REG gamma deficiency results in dose-dependent reduction in litter size.