Synergistic effects of metal nanoparticles and a phenolic uncoupler using microdroplet-based two-dimensional approach
Synergistic effects of metal nanoparticles and a phenolic uncoupler using microdroplet-based two-dimensional approach
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DOI：
10.1039/c0em00162g
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发表时间：
2011-02-01
影响因子：
--
通讯作者：
Koehler, J. Michael
中科院分区：
文献类型：
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作者：
Funfak, Anette;Cao, Jialan;Koehler, J. Michael
提出了一种用于测试多种试剂浓度的基于液滴的微流体技术。我们使用这种实验方法来研究金（AuNP）和银纳米粒子（AgNP）与酚解偶联剂2，4-二硝基苯酚（DNP）的组合效应与大肠杆菌的生长。为了评价二元混合物的毒性，我们首先将E.使用PEEK（聚醚酮）模块在微滴内对大肠杆菌细胞和颗粒混合物进行了测试。二维浓度空间约500以及分离液滴解决。随后，我们用微流通荧光计和光度计分析了细菌的细胞生长、活力和自发荧光强度（代谢活性）。发现AgNPs和DNP的二元混合物具有剂量依赖性协同效应，这表明混合物中的相互作用比效应总和预期的更强。对于无毒浓度的DNP和AuNP的二元混合物，我们发现在低DNP浓度下仅存在弱的协同效应。此外，无毒的测试的AuNP在具有酚类解偶联剂的二元混合物中引起效应总和。在一般情况下，我们证明了基于液滴的微流体系统的效率，用于快速高通量筛选二元和多元混合物。这项工作还证实了高分辨率液滴为基础的测定生态毒理学水生测试系统的小型化的相关性。
A droplet-based microfluidic technique for testing multiple reagent concentrations is presented. We used this experimental approach to study combined effects of gold (AuNP) and silver nanoparticles (AgNP) with the phenolic uncoupler 2,4-dinitrophenol (DNP) with respect to the growth of Escherichia coli. In order to evaluate the toxicity of binary mixtures, we first encapsulated the E. coli cells and particle mixtures inside the microdroplets using PEEK (polyetherketone) modules. Two-dimensional concentration spaces with about 500 well separated droplets were addressed. We subsequently analyzed the cell growth, the viability and the autofluorescence intensity (metabolic activity) of the bacteria with a micro-flow-through fluorometer and photometer. Dose-dependent synergistic effects were found for the binary mixture of AgNPs and DNP, which indicated a stronger interaction in the mixture than it was expected from effect summation. For the binary mixture of DNP and AuNPs in non-toxic concentrations, we found only weak synergistic effects at low DNP concentrations. Furthermore, the non-toxic tested AuNPs causes effect summation in the binary mixture with the phenolic uncoupler. In general, we demonstrated the efficiency of a droplet-based microfluidic system for fast high-throughput screenings of binary and multiple mixtures. This work also confirmed the relevance of highly resolved droplet-based assays for the miniaturization of ecotoxicological aquatic test systems.