Genome-wide identification of and functional insights into the late embryogenesis abundant (LEA) gene family in bread wheat (Triticum aestivum)
Genome-wide identification of and functional insights into the late embryogenesis abundant (LEA) gene family in bread wheat (Triticum aestivum)
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面包小麦 (Triticum aestivum) 晚期胚胎发生丰富 (LEA) 基因家族的全基因组鉴定和功能洞察
DOI：
10.1038/s41598-019-49759-w
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发表时间：
2019-09-16
影响因子：
4.6
通讯作者：
Zhang, Linsheng
中科院分区：
文献类型：
--
作者：
Liu, Hao;Xing, Mingyan;Zhang, Linsheng
晚期胚胎发生丰富（LEA）蛋白参与植物对各种非生物胁迫的响应和适应，包括脱水、盐度、高温和寒冷。在此，我们报告了对“中国春”小麦（Triticum aestivum）LEA 基因家族的首次全面调查。在普通小麦中共鉴定出 179 个 TaLEA 基因，并分为 8 组。所有 TaLEA 基因都含有 LEA 保守基序并且几乎没有内含子。属于同一组的TaLEA基因表现出相似的基因结构和染色体位置。我们的结果表明，大多数 TaLEA 基因在启动子区含有脱落酸（ABA）响应元件（ABRE）和各种与胁迫反应相关的顺式作用元件，并在 ABA 和非生物胁迫处理下被诱导。此外，将代表每组的8个基因引入大肠杆菌和酵母中，以研究TaLEAs在热和盐胁迫下的保护功能。 TaLEAs 增强了大肠杆菌和酵母对盐和热的耐受性，表明这些蛋白质在应激条件下对宿主细胞具有保护功能。这些结果增加了我们对 LEA 基因的理解，并为未来旨在提高小麦抗逆性的功能研究提供了强有力的候选基因。
Late embryogenesis abundant (LEA) proteins are involved in the responses and adaptation of plants to various abiotic stresses, including dehydration, salinity, high temperature, and cold. Here, we report the first comprehensive survey of the LEA gene family in "Chinese Spring" wheat (Triticum aestivum). A total of 179 TaLEA genes were identified in T. aestivum and classified into eight groups. All TaLEA genes harbored the LEA conserved motif and had few introns. TaLEA genes belonging to the same group exhibited similar gene structures and chromosomal locations. Our results revealed that most TaLEA genes contained abscisic acid (ABA)-responsive elements (ABREs) and various cis-acting elements associated with the stress response in the promoter region and were induced under ABA and abiotic stress treatments. In addition, 8 genes representing each group were introduced into E. coli and yeast to investigate the protective function of TaLEAs under heat and salt stress. TaLEAs enhanced the tolerance of E. coli and yeast to salt and heat, indicating that these proteins have protective functions in host cells under stress conditions. These results increase our understanding of LEA genes and provide robust candidate genes for future functional investigations aimed at improving the stress tolerance of wheat.