Computational framework for analyzing flow-induced strain on osteocyte as modulated by microenvironment
Computational framework for analyzing flow-induced strain on osteocyte as modulated by microenvironment
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DOI：
10.1016/j.jmbbm.2021.105027
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发表时间：
2022-02-01
影响因子：
3.9
通讯作者：
Adachi, Taiji
中科院分区：
文献类型：
--
作者：
Kameo, Yoshitaka;Ozasa, Masahiro;Adachi, Taiji
埋藏在骨基质中的骨细胞是主要的机械感觉细胞，其响应于腔隙-小管孔隙中的间质液流动来调节骨重建。为了了解骨细胞机械感知的机制，重要的是能够评估由间质液流动引起的骨细胞突起膜上的局部应变。骨细胞的微环境，包括细胞周基质（PCM）和小管超微结构，是骨细胞突起膜上的流动诱导应变的关键调节剂，因为它在细胞周空间中产生不均匀的流动模式。为了研究骨细胞微环境的变化对流动诱导应变的影响，我们开发了一种新的计算框架来分析流固耦合。计算机模拟的基础上提出的框架，使骨细胞突起膜上的流动诱导的应变的空间分布的评价，根据PCM密度和小管曲率的变化。模拟结果表明，PCM密度的降低和小管曲率的增加，其中每一个都与衰老和骨骼疾病相关，具有显著的增强局部流动诱导的应变对骨细胞突起膜的影响。我们相信，拟议的计算框架是一个很有前途的框架，调查细胞特异性的机械刺激，它有可能加速骨细胞的机械生物学研究，提供更深入的了解他们的机械环境在活骨组织。
Osteocytes buried in bone matrix are major mechanosensory cells that regulate bone remodeling in response to interstitial fluid flow in a lacuno-canalicular porosity. To gain an understanding of the mechanism of osteocyte mechanosensing, it is important to be able to evaluate the local strain on the osteocyte process membrane induced by the interstitial fluid flow. The microenvironment of the osteocytes, including the pericellular matrix (PCM) and canalicular ultrastructure, is a key modulator of the flow-induced strain on the osteocyte process membrane because it produces heterogeneous flow patterns in the pericellular space. To investigate the effect of changes in the microenvironment of osteocytes on the flow-induced strain, we developed a novel computational framework for analyzing the fluid-structure interaction. Computer simulations based on the proposed framework enabled evaluation of the spatial distribution of flow-induced strain on the osteocyte process membrane according to changes in the PCM density and canalicular curvature. The simulation results reveal that a decrease in PCM density and an increase in canalicular curvature, each of which is associated with aging and bone disease, have the notable effect of enhancing local flow-induced strain on the osteocyte process membrane. We believe that the proposed computational framework is a promising framework for investigating cell-specific mechanical stimuli and that it has the potential to accelerate the mechanobiological study of osteocytes by providing a deeper understanding of their mechanical environment in living bone tissue.