Vimentin-targeting AIEgen-peptide conjugates: Wash-free fluorescence detection of EMT-type cancer cells and tissues
Vimentin-targeting AIEgen-peptide conjugates: Wash-free fluorescence detection of EMT-type cancer cells and tissues
复制标题
波形蛋白靶向 AIEgen-肽缀合物：EMT 型癌细胞和组织的免洗荧光检测
DOI：
10.1016/j.snb.2020.128536
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Kan Quancheng
中科院分区：
文献类型：
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作者：
Wei Han;Yu Yifang;Wang Yueqin;Ying Zhenzhen;Cheng Weiyan;Tian Xin;Kan Quancheng
上皮-间质转化（EMT）型癌细胞与多种肿瘤的化疗耐药、转移和复发有关。波形蛋白是一种在肿瘤转移过程中起重要作用的细胞骨架蛋白，在EMT型细胞中高表达。目前，用于检测EMT型癌细胞的流行免疫测定是昂贵且不方便的，并且仍然需要可以简单且灵敏地鉴定这种重要类型的细胞的新方法。在这项工作中，我们设计并合成了一对pureZ/E异构体的波形蛋白靶向TPE-肽缀合物。研究了这些立体异构探针的光学特性差异。此外，通过使用这些探针的免洗涤荧光检测，成功地开发了用于方便和选择性检测EMT型癌细胞和组织的方法。
Epithelial-mesenchymal transitions (EMT)-type cancer cells are related to chemoresistance, metastasis and tumor relapse in various kinds of cancers. Vimentin is one kind of cytoskeletal proteins that play important roles during cancer metastasis and high-expressed in EMT-type cells. Presently, prevalent immunoassays for detecting EMT-type cancer cells are expensive and inconvenient, and novel methods that can simply and sensitively identify cells of this vital type are still needed. In this work, we designed and synthesized a pair of pureZ/Eisomers of vimentin-targeting TPE-peptide conjugates. The difference of optical features between these stereoisomeric probes was investigated. Furthermore, methods for convenient and selective detection of EMT-type cancer cells and tissues were successfully developed through wash-free fluorescence detection using these probes.