Common molecular mechanisms in field- and agrin-induced acetylcholine receptor clustering.
Common molecular mechanisms in field- and agrin-induced acetylcholine receptor clustering.
复制标题
场和集聚蛋白诱导的乙酰胆碱受体聚集的常见分子机制。
DOI：
10.1023/a:1026365812496
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发表时间：
1997
影响因子：
4
通讯作者：
Stollberg,J
中科院分区：
文献类型：
--
作者：
Sabrina,F;Stollberg,J
1.乙酰胆碱受体在发育中的神经肌肉接头处的聚集对该突触的发育和功能至关重要，体外研究表明聚集蛋白在肌细胞表面的发现和电场诱导的非受体分子在阴极细胞极的聚集可以诱导受体聚集.我们在这里报告的聚集蛋白结合和电场之间的相互作用的受体和聚集蛋白结合位点的分布。(a)用聚集蛋白预处理细胞完全阻断场诱导的受体簇的发展。(b)场诱导的受体聚集先于场诱导的聚集蛋白结合位点聚集约30分钟。(c)电场阻止聚集蛋白诱导的受体聚集，尽管存在聚集蛋白结合位点和自由扩散的受体.这些结果表明，另一个膜组件，但不是聚集蛋白结合位点，而不是受体，是需要聚集蛋白诱导的受体聚集。他们还表明，电场和聚集蛋白通过共同的分子机制引起受体聚集。
1. The aggregation of acetylcholine receptors at the developing neuromuscular junction is critical to the development and function of this synapse.In vitrostudies have shown that receptor aggregation can be induced by the finding of agrin to the muscle cell surface and by the electric field-induced concentration of a (nonreceptor) molecule at the cathodal cell pole.2. We report here on the interaction between agrin binding and electric fields with respect to the distribution of receptors and agrin binding sites.3. (a) Pretreatment of cells with agrin completely blocks the development of field-induced receptor clusters. (b) Field-induced aggregation of receptors precedes the field-induced aggregation of agrin binding sites by approximately 30min. (c) Electric fields prevent agrin-induced receptor clustering despite the presence of agrin binding sites and freely diffusing receptors.4. These results indicate that another membrane component—but not the agrin binding site and not the receptor—is required for agrin-induced receptor clustering. They also suggest that electric fields and agrin cause receptor clustering via common molecular mechanisms.