Exploring frontiers of orbitrap performance for long transients
Exploring frontiers of orbitrap performance for long transients
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DOI：
10.1016/j.ijms.2021.116607
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发表时间：
2021-05-04
影响因子：
1.8
通讯作者：
Makarov, Alexander
中科院分区：
文献类型：
--
作者：
Denisov, Eduard;Damoc, Eugen;Makarov, Alexander
在改进的 Orbitrap Exploris 仪器上进行的实验表明，即使在紧凑型仪器中，当满足适当的容差和调谐要求时，也可以实现超过 2,000,000 的分辨率。检测时间为 4 秒即可达到这样的水平。采用改进的泵送的新 Orbitrap 设计首次允许完整单克隆抗体的同位素分辨率，即使在离子路由多极杆中使用标准氮气供应时也是如此。更长的瞬态进一步提高了信噪比，接近电荷检测质谱中单电荷分辨率所需的水平。讨论了改进仪器的进一步步骤。(c) 2021 Elsevier B.V. 保留所有权利。
Experiments on a modified Orbitrap Exploris instrument have shown that even in a compact instrument it is possible to achieve a resolving power in excess of 2,000,000 when appropriate tolerance and tuning requirements are met. Such levels are achieved for a 4-s detection time.A new Orbitrap design with improved pumping allowed, for the first time, isotopic resolution of intact monoclonal antibodies even when using standard nitrogen supply in the Ion Routing Multipole. Longer transients further improve signal-to-noise ratios, approaching levels needed for single-charge resolution in charge detection mass spectrometry. Further steps for improvement of instrumentation are discussed.(c) 2021 Elsevier B.V. All rights reserved.