Time course of Fos and Fras expression in the hypothalamic supraoptic neurons during chronic osmotic stimulation
Time course of Fos and Fras expression in the hypothalamic supraoptic neurons during chronic osmotic stimulation
复制标题
DOI：
10.1016/s0169-328x(01)00072-9
复制
发表时间：
2001-05-20
期刊：
影响因子：
--
通讯作者：
Kiyohara, T
中科院分区：
文献类型：
--
作者：
Miyata, S;Tsujioka, H;Kiyohara, T
Fos家族包括Fos和Fos相关蛋白（Fras）的几种亚型，如FosB、Fra-1、Fra-2、Delta FosB和慢性Fras。Fos家族蛋白表达随时间的变化还没有很好地阐明，特别是在慢性刺激期间。在本实验中，我们定量研究了急性和慢性渗透刺激时视上核（SON）大细胞神经元Fos，FosB和Fras免疫反应的时程变化。在对照组大鼠的SON中观察到少量Fos和FosB阳性神经元，而在对照组动物中观察到许多Fras阳性神经元。在急性渗透压刺激后2 h，观察到Fos-、FosB-和Fras-阳性神经元的数量显著增加。注射3%NaCl溶液。虽然在腹腔注射后4 h Fos阳性神经元的数量恢复到对照水平，但在腹腔注射后8 h仍观察到显著数量的FosB和Fras阳性神经元。在2% NaCl溶液慢性渗透刺激2 d和5 d时，可观察到大量Fos阳性神经元，而正常饮水停止慢性渗透刺激后，Fos阳性神经元数量立即下降，在2 h内降至对照水平。FosB阳性神经元的数量随着慢性渗透刺激时间的延长而增加，在刺激停止后2-8 h观察到大量的FosB阳性神经元。在慢性渗透刺激期间，Fras阳性神经元的数量也显著较高，并且在刺激停止后2-8 h，该数量显著较高。RT-PCR结果显示，慢性渗透压刺激可使视上核c-fos mRNA持续表达。这些结果表明，c-fos mRNA和Fos蛋白在慢性渗透刺激过程中组成性升高，Fos的时程变化不同于FosB和Fras。(C)2001 Elsevier Science B. V.保留所有权利。
The Fos family comprises Fos and several subtypes of Fos-related proteins (Fras) such as FosB, Fra-1, Fra-2, Delta FosB, and chronic Fras. Changes in the expression of Fos family proteins with time are not well elucidated, particularly during chronic stimulation. In the present experiments, we investigated quantitatively the time course changes in Fos, FosB and Fras immunoreactivity in the magnocellular neurons of the supraoptic nucleus (SON) during acute and chronic osmotic stimulation. A small number of Fos- and FosB-positive neurons were observed in the SON of control rats, while many Fras-positive neurons were seen in control animals. Significant increases in the numbers of Fos-, FosB-, and Fras-positive neurons were observed 2 h after acute osmotic stimulation by intraperitoneal (i.p.) injection of 3% NaCl solution. Although the number of Fos-positive neurons returned to the control level 4 h after i.p. injection, a significant number of FosB- and Fras-positive neurons were still observed 8 h after i.p. injection. During chronic osmotic stimulation by giving 2% NaCl solution for 2 and 5 days, a large number of Fos-positive neurons were observed, but the cessation of chronic osmotic stimulation by normal water drinking immediately decreased the number of Fos-positive neurons to the control level within 2 h. The number of FosB-positive neurons was increased with period of chronic osmotic stimulation, and a significant number were observed 2-8 h after the cessation of the stimulation. The number of Fras-positive neurons was also significantly higher during chronic osmotic stimulation, and this number was significantly high 2-8 h after the cessation of the stimulation. RT-PCR analysis demonstrated the persistent expression of c-fos mRNA in the SON during chronic osmotic stimulation. These results suggest that c-fos mRNA and Fos protein are constitutively elevated during chronic osmotic stimulation and the time course changes in Fos are different from those seen in FosB and Fras. (C) 2001 Elsevier Science B.V. All rights reserved.