Non‐muscle myosin as target antigen for human autoantibodies in patients with hepatitis C virus‐associated chronic liver diseases
Non‐muscle myosin as target antigen for human autoantibodies in patients with hepatitis C virus‐associated chronic liver diseases
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DOI：
10.1111/j.1365-2249.1995.tb03605.x
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发表时间：
1995-04
影响因子：
4.6
通讯作者：
C. A. Mühlen;E. Chan;C. Peebles;H. Imai;K. Kiyosawa;E. Tan
中科院分区：
文献类型：
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作者：
C. A. Mühlen;E. Chan;C. Peebles;H. Imai;K. Kiyosawa;E. Tan
3例丙型肝炎病毒（HCV）相关慢性肝病患者显示自身抗体与非肌肉细胞的细胞骨架纤维强烈反应。非肌肉肌球蛋白微丝的重链是这些自身抗体的主要靶点，这是由(i)细胞和组织免疫荧光研究确定的，显示了与抗肌球蛋白抗体原型的共定位；（ii）使用MOLT‐4细胞、人血小板或亲和纯化的非肌肉肌球蛋白作为抗原提取物，用200‐kD蛋白进行免疫印迹的初级反应性；（iii）利用这些人血清和抗体原型，对木瓜蛋白酶消化的MOLT - 4细胞提取物中类似的免疫反应片段进行免疫印迹。非肌肉肌球蛋白重链自身抗体在慢性肝病患者中未见报道，特别是在与HCV感染相关的患者中。
Three patients with hepatitis C virus (HCV)‐related chronic liver disease were shown to have autoantibodies strongly reacting with cytoskeletal fibres of non‐muscle cells. The heavy chain of non‐muscle myosin microfilament was the main target for those autoantibodies, as determined by (i) cell and tissue immunofluorescence studies showing colocalization with an anti‐myosin antibody prototype; (ii) primary reactivity in immunoblotting with a 200‐kD protein, using either MOLT‐4 cells, human platelets, or affinity‐purified non‐muscle myosin as antigen extract; and (iii) immunoblotting of similar immunoreactive fragments in papain‐digested MOLT‐4 cell extracts, by using those human sera and antibody prototype. Autoantibodies to non‐muscle myosin heavy chain were not previously reported in patients with chronic liver diseases, especially in those associated with HCV infection.