Effects of exhaustive exercises, with different intensities, on oxidative stress markers in rat plasma and skeletal muscle
Effects of exhaustive exercises, with different intensities, on oxidative stress markers in rat plasma and skeletal muscle
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DOI：
10.1016/j.scispo.2017.08.008
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发表时间：
2018-06-01
期刊：
影响因子：
1.1
通讯作者：
Muraoka, I
中科院分区：
文献类型：
--
作者：
Kawamura, T.;Fujii, R.;Muraoka, I
目标。 - 本研究的目的是检查不同强度的力竭运动对大鼠血浆和骨骼肌中氧化应激标记物的影响。方法。 -雄性Sprague-Dawley大鼠(n=24)被随机分为以下四组：对照组(CON)、低强度运动(LE)、高强度间歇运动(HE)和增量运动(IE)。后三组动物进行跑步至力竭的练习如下： LE组动物以20m/min（6度分级）跑步； HE组以40 m/min的速度跑30 s（0度分级），每次跑之间休息60 s； IE组最初以15 m/min（6度分级）跑步，之后逐渐增加跑步速度，每10 min增加5 m/min。运动结束后立即抽取血样，然后迅速取出腓肠肌和肝脏。分析这些样品的乳酸浓度、氧化应激标记物和糖原含量。结果。 - LE和HE组的血浆氧化应激标志物没有变化，而IE组的血浆蛋白羰基（PC）水平和总抗氧化能力（TAC）与CON组相比显着升高（P < 0.05）。相反，三个运动组骨骼肌中的所有氧化应激标志物与 CON 组相比没有变化。结论。 - 增量力竭运动会升高血浆 PC 水平和 TAC，但不会改变大鼠骨骼肌氧化应激标志物。 (C) 2017 Elsevier Masson SAS。版权所有。
Objectives. - The purpose of this study was to examine the effects of exhaustive exercises, with different intensities, on oxidative stress markers in rat plasma and skeletal muscle.Methods. - Male Sprague-Dawley rats (n=24) were randomly divided into the following four groups: control (CON), low-intensity exercise (LE), high-intensity interval exercise (HE) and incremental exercise (IE). The animals in the latter three groups performed exercises in which they ran until exhaustion as follows: Animals in the LE group ran at 20 m/min (6 degrees graded); the HE group ran at 40 m/min for 30 s (0 degrees graded) with a rest interval of 60 s between each run; and the IE group ran at 15 m/min (6 degrees graded), initially, with the running speed gradually increased by 5 m/min, every 10 min. Immediately following the exercise, a blood sample was drawn, and followed by the gastrocnemius muscle and liver were quickly removed. These samples were analysed for lactate concentration, oxidative stress markers, and glycogen contents.Results. - Plasma oxidative stress markers in the LE and HE groups demonstrated no change, while plasma protein carbonyl (PC) levels and total antioxidant capacity (TAC) in the IE group were significantly increased compared with the CON group (P < 0.05). In contrast, all the oxidative stress markers, in the skeletal muscle of the three exercise groups, demonstrated no change compared with the CON group.Conclusion. - Incremental exhaustive exercise elevates the plasma PC levels and TAC, but does not change skeletal muscle oxidative stress markers in rats. (C) 2017 Elsevier Masson SAS. All rights reserved.