Metabonomic study of biochemical changes in the urine of Morning Glory Seed treated rat
Metabonomic study of biochemical changes in the urine of Morning Glory Seed treated rat
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牵牛花籽处理大鼠尿液生化变化的代谢组学研究
DOI：
10.1016/j.jpba.2010.03.034
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发表时间：
2010-11-02
影响因子：
3.4
通讯作者：
Chen, Xiaohui
中科院分区：
文献类型：
--
作者：
Ma, Chao;Bi, Kaishun;Chen, Xiaohui
本文旨在研究治疗水肿、单纯性肥胖和肺热的中药牵牛花种子（MGS）致肾毒性的代谢组学特征。采用超高效液相色谱/质谱法（UPLC-MS）在正离子模式下对对照组和MGS处理的大鼠尿液样本进行分析。通过血液生化和组织病理学检查来确定肾损害的具体变化。结果表明，用乙醇提取物（EMGS）代替水提取物（WMGS），UPLC-MS分析显示，EMGS处理大鼠尿液中8种内源性代谢物作为生物标志物的水平发生了显著变化，并根据所鉴定的代谢物讨论了EMGS干扰代谢途径的潜在调控，本研究证明了基于UPLC-MS的代谢组学在绘制代谢反应毒理学方面的潜力。(c) 2010 Elsevier B V版权所有
This paper was designed to study metabonomic characters of the nephrotoxicity induced by Morning Glory Seed (MGS), a well-known traditional Chinese medicine which was used for the treatment of edema, simple obesity and lung fever. Urinary samples from control and MGS treated rats were analyzed by ultra-performance liquid chromatography/mass spectrometry (UPLC-MS) in positive ionization mode. Blood biochemistry and histopathology were examined to identify specific changes of renal damage. The results affirmatively suggested that ethanol extract of Morning Glory Seed (EMGS), instead of water extract of Morning Glory Seed (WMGS), should be responsible for the nephrotoxicity caused by this herbal medicine The UPLC-MS analysis revealed that the levels of 8 endogenous metabolites as biomarkers were significantly changed in urine from EMGS treated rats The underlying regulations of EMGS-perturbed metabolic pathways were discussed according to the identified metabolites The present study proves the potential of UPLC-MS based metabonomics in mapping metabolic response for toxicology. (c) 2010 Elsevier B V All rights reserved