Zebrafish Nk-lysins: First insights about their cellular and functional diversification
Zebrafish Nk-lysins: First insights about their cellular and functional diversification
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DOI：
10.1016/j.dci.2015.03.009
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发表时间：
2015-07-01
影响因子：
2.9
通讯作者：
Novoa, B.
中科院分区：
文献类型：
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作者：
Pereiro, P.;Varela, M.;Novoa, B.
Nk-lysins是由细胞毒性T淋巴细胞和自然杀伤细胞产生的抗菌蛋白，具有广泛的抗菌谱（包括细菌、真菌和寄生虫）。然而，这些蛋白质在防止病毒感染方面的含义仍然知之甚少。在这项工作中，在斑马鱼基因组中鉴定出四种不同的Nk-lysin基因（nkla, nklb， nklc和nkld）。这意味着斑马鱼是迄今为止所描述的具有较高Nk-lysin基因库的物种。Nk-lysin在不同组织、个体发育过程中、不同肾细胞群之间以及Rag1（-/-）和Rag1（+/+）个体之间的差异表达模式，可能表明不同细胞类型在不同Nk-lysin的产生中具有一定的特化。此外，这些基因中只有两个（nkla和nkld）在病毒感染后显著上调，这一观察结果也可能是斑马鱼Nk-lysins功能多样化的结果。(C) 2015 Elsevier Ltd.版权所有。
Nk-lysins are antimicrobial proteins produced by cytotoxic T lymphocytes and natural killer cells with a broad antimicrobial spectrum (including bacteria, fungi and parasites). Nevertheless, the implication of these proteins in the protection against viral infections is still poorly understood. In this work, four different Nk-lysin genes (nkla, nklb, nklc and nkld) were identified in the zebrafish genome. That means that zebrafish is the species with the higher repertoire of Nk-lysin genes described so far. The differential expression pattern of the Nk-lysins in several tissues, during ontogeny, among the different kidney cell populations, as well as between Rag1(-/-) and Rag1(+/+) individuals, could suggest a certain specialization of different cell types in the production of different Nk-lysin. Moreover, only two of these genes (nkla and nkld) were significantly up-regulated after viral infection, and this observation could be also a consequence of a functional diversification of the zebrafish Nk-lysins. (C) 2015 Elsevier Ltd. All rights reserved.