BMP-6 enhances chondrogenesis in a subpopulation of human marrow stromal cells
BMP-6 enhances chondrogenesis in a subpopulation of human marrow stromal cells
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DOI：
10.1006/bbrc.2001.4898
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发表时间：
2001-06-08
影响因子：
3.1
通讯作者：
Prockop, DJ
中科院分区：
文献类型：
--
作者：
Sekiya, I;Colter, DC;Prockop, DJ
骨髓基质细胞（MSC）可以分化成几种间充质谱系，最近显示MSC在含有TGF-β和地塞米松的无血清培养基的微团培养物中形成软骨。在这里，我们发现添加BMP-6使颗粒的重量增加约10倍，并且它们对蛋白聚糖的染色更广泛，在1周时检测到II型前胶原和X型胶原的mRNA，并且在3周时水平增加。我们还比较了两种MSC培养物亚群：小型快速自我更新细胞（RS细胞）和大型更成熟且复制缓慢的细胞（mMSC）。从富含RS细胞的培养物制备的软骨颗粒约大2.5倍，蛋白多糖染色更广泛，II型前胶原的mRNA水平高1.6倍。此外，随着细胞传代，RS细胞保留更多的软骨形成潜力。(C)北京：清华大学出版社.
Marrow stromal cells (MSCs) can differentiate into several mesenchymal lineages, MSCs were recently shown to form cartilage in micromass cultures with serum-free medium containing TGF-beta and dexamethasone, Here we found that addition of BMP-6 increased the weight of the pellets about 10-fold and they stained more extensively for proteoglycans, mRNAs for type II procollagen and type X collagen were detected at 1 week and the levels were increased at 3 weeks. We also compared two subpopulation of cultures of MSCs: Small and rapidly self-renewing cells (RS cells) and the large, more mature and slowly replicating cells (mMSCs). The cartilage pellets prepared from cultures enriched for RS cells were about 2.5-fold larger, stained more extensively for proteoglycans, and had levels of mRNA for type II procollagen that were 1.6-fold higher, Also, RS cells retained more of their chondrogenic potential as the cells were passaged. (C) 2001Academic Press.