A Bombyx mandarina mutant exhibiting translucent larval skin is controlled by the molybdenum cofactor sulfurase gene
A Bombyx mandarina mutant exhibiting translucent larval skin is controlled by the molybdenum cofactor sulfurase gene
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DOI：
10.1266/ggs.84.147
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发表时间：
2009-04-01
影响因子：
1.1
通讯作者：
Shimada, Toru
中科院分区：
文献类型：
--
作者：
Fujii, Tsuguru;Ozaki, Masataka;Shimada, Toru
在野蚕（Bombyx mandarina）的饲养过程中，发现了一种表现出半透明皮肤的突变表型。通过与家蚕Bombyx mori的杂交实验，我们发现该突变体受钼辅因子硫酸酶（MoCoS）基因控制。我们将突变体命名为“尾崎半透明”（og(Z)）。我们发现了一个 2.1 kb 的缺失，其中包含 MoCoS 基因的转录起始位点、外显子 1 和 2 以及外显子 3 的 5' 末端。在 og(Z) 纯合子中未检测到 MoCoS 基因的转录本。我们得出结论，og(Z) 是由 MoCoS 基因破坏产生的完全功能丧失的等位基因。
During the maintenance of the wild silkworm, Bombyx mandarina, a mutant phenotype exhibiting translucent skin was identified. Based on the crossing experiments with the domesticated silkworm, Bombyx mori, we found that the mutant was controlled by molybdenum cofactor sulfurase (MoCoS) gene. We designated the mutant "Ozaki's translucent" (og(Z)). We found a 2.1-kb deletion containing the transcription initiation site, exons 1 and 2, and the 5' end of exon 3 of the MoCoS gene. The transcript of the MoCoS gene was not detected in the og(Z) homozygote. We concluded that og(Z) is a complete loss-of-function allele generated by a disruption of the MoCoS gene.