The physiological and behavioral effects of carbon dioxide on Drosophila melanogaster larvae
The physiological and behavioral effects of carbon dioxide on Drosophila melanogaster larvae
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DOI：
10.1016/j.cbpb.2005.01.019
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发表时间：
2005-03-01
影响因子：
2.3
通讯作者：
Cooper, RL
中科院分区：
文献类型：
--
作者：
Badre, NH;Martin, ME;Cooper, RL
成虫和幼虫被二氧化碳（CO2）迅速麻醉;然而，其机制尚未得到解决。在这项研究中，我们使用果蝇幼虫调查的行动，以解释快速制动和心脏骤停与急性暴露于CO2的行为影响。为了确定是否需要中枢神经系统（CNS），进行了有和无CNS的研究。本文还研究了CO_2暴露引起的低pH效应。与含CO2的生理盐水相比，酸性生理盐水会增加心率。在骨骼肌神经肌肉接头（NMJ）的突触传递被CO2阻断，但不是由低pH值。该网站的行动是突触后谷氨酸，神经递质在果蝇NMJ的敏感性降低。当暴露于CO2时，CNS在突触传递中保持活跃，这与NMJ处的突触形成对比。总之，CO2的影响直接介导心脏停止，并通过降低对运动神经末梢释放的神经递质谷氨酸的敏感性来介导骨骼NMJ。快速的行为和生理影响不能占的行动中枢神经系统内的幼虫，也不是由CO2间接诱导的pH值的影响。D.黑腹动物制备物在功能上与脊椎动物中的离子型谷氨酸受体相似，这可以解释脊椎动物中尚未从机理上解释的CO2的观察现象。(c)2005年爱思唯尔公司All rights reserved.
Adult and larval insects are rapidly anesthetized by carbon dioxide (CO2); however, the mechanisms have not been addressed. In this study, we use larval Drosophila to investigate the actions of CO2 to explain the behavioral effects of rapid immobilization and cardiac arrest with acute exposure to CO2. To determine if the central nervous system (CNS) is required, studies were performed with and without the CNS. The effects of low pH induced by exposure to CO2 Were also examined. An acidic saline increases the heart rate in contrast to saline containing CO2. Synaptic transmission at the skeletal neuromuscular junction (NMJ) is blocked by CO2 but not by low pH. The site of action is postsynaptic by a decreased sensitivity to glutamate, the neurotransmitter at Drosophila NMJs. The CNS remains active in synaptic transmission when exposed to CO2 which is in contrast to the synapses at the NMJ. In summary, the effects of CO2 are directly mediated on the heart to stop it and at skeletal NMJs by a reduced sensitivity to glutamate, the released neurotransmitter, from the motor nerve terminals. The rapid behavioral and physiological effects cannot be accounted for by action on the CNS within the larvae nor by a pH effect indirectly induced by CO2. The glutamate receptors in the D. melanogaster preparation are similar in function to ionotropic glutamate receptors in vertebrates which could account for the observational phenomena of CO2 not yet explained mechanistically in vertebrates. (c) 2005 Elsevier Inc. All rights reserved.