Regulation of compound leaf development in mungbean (Vigna radiata L.) by CUP-SHAPED COTYLEDON/NO APICAL MERISTEM (CUC/NAM) gene
Regulation of compound leaf development in mungbean (Vigna radiata L.) by CUP-SHAPED COTYLEDON/NO APICAL MERISTEM (CUC/NAM) gene
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DOI：
10.1007/s00425-018-3038-z
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发表时间：
2019-03-01
期刊：
影响因子：
4.3
通讯作者：
Shen, Zhenguo
中科院分区：
文献类型：
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作者：
Jiao, Keyuan;Li, Xin;Shen, Zhenguo
结论该结果验证了豆科植物CUC/NAM基因的功能冗余性和多样性。杯形子叶/无顶分生组织（CUC/NAM）同源物在植物器官边界形成和器官发育中起着关键作用。在这里，我们对绿豆的γ辐照诱变群体进行了正向筛选，并鉴定了一个突变体，减少轴和融合小叶（rrf1），该突变体产生了减少轴和融合小叶的复叶。图谱克隆显示，RRF1在绿豆中编码CUC/NAM蛋白。系统发育分析表明，豆科植物的CUC1/CUC2基因可分为两个亚支系，且在豆科植物中存在不同拷贝数。转录组学分析显示，与野生型植物相比，rrf1突变体中一系列发育调控因子的表达水平发生了改变，包括I类KNOXI基因和侧器官边界域（LBD）基因。此外，rrf1与七叶复叶突变体hel1发生遗传互作，调控绿豆叶片发育。我们的研究结果表明，在一个祖先基因的重复之后，豆科植物中CUC1/CUC2基因的两个亚支系存在功能冗余和多样性。
Main conclusionThe results provide a significant verification of functional redundancy and diversity of CUC/NAM genes in legumes.The CUP-SHAPED COTYLEDON/NO APICAL MERISTEM (CUC/NAM) orthologs play key roles for plant organ boundary formation and organ development. Here, we performed a forward screen of the gamma irradiation mutagenesis population in mungbean and characterised a mutant, reduced rachis and fused leaflets (rrf1), which gave rise to the formation of compound leaves with reduced rachis and fused leaflets. Map-based cloning revealed that RRF1 encoded a CUC/NAM protein in mungbean. Phylogenetic analysis indicated that legume CUC1/CUC2 genes were classified as belonging to two subclades, and there are different copies of CUC1/CUC2 genes in legumes. Transcriptomic analysis showed that expression levels of a set of developmental regulators, including class I KNOTTED-LIKE HOMEOBOXI (KNOXI) gene and LATERAL ORGAN BOUNDARIES DOMAIN (LBD) gene, were altered in rrf1 mutants compared to the wild-type plants. Furthermore, rrf1 genetically interacted with heptafoliate leaflets1 (hel1), a mutant displaying a seven-leaflet compound leaf, to regulate leaf development in mungbean. Our results suggest functional redundancy and diversity of two subclades of CUC1/CUC2 genes in legumes, following the duplication of an ancestral gene.