A time-resolved luminescence biosensor assay for anaplastic lymphoma kinase (ALK) activity
A time-resolved luminescence biosensor assay for anaplastic lymphoma kinase (ALK) activity
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DOI：
10.1039/c4cc07453j
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发表时间：
2015-01-01
影响因子：
4.9
通讯作者：
Parker, Laurie L.
中科院分区：
文献类型：
--
作者：
Cui, Wei;Parker, Laurie L.
建立了一种新型的间变性淋巴瘤激酶(ALK)时间分辨发光生物传感器检测方法。我们使用了一种简单的策略将已知的ALK底物修饰成一种能够适应被ALK磷酸化后的Tb发光敏化的多肽生物传感器。由于这一策略是可推广的，这种高通量筛查兼容分析为开发其他使用Tb发光作为读出的激酶分析提供了一个范例。
A novel time-resolved luminescence biosensor assay for anaplastic lymphoma kinase (ALK) was developed. We used a straightforward strategy to modify a known ALK substrate into a peptide biosensor that can accommodate terbium luminescence sensitization upon its phosphorylation by ALK. Since this strategy is generalizable, this high-throughput screening compatible assay serves as an example for development of other kinase assays that employ terbium luminescence as a read-out.