Polyamine-metabolizing enzymes are activated to promote the proper assembly of rice stripe mosaic virus in insect vectors.
Polyamine-metabolizing enzymes are activated to promote the proper assembly of rice stripe mosaic virus in insect vectors.
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多胺代谢酶被激活以促进水稻条纹花叶病毒在昆虫载体中的正确组装
DOI：
10.1007/s44154-021-00032-z
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发表时间：
2022-04-15
期刊：
影响因子：
--
通讯作者：
Wei T
中科院分区：
文献类型：
--
作者：
Jia D;Liu H;Zhang J;Wan W;Wang Z;Zhang X;Chen Q;Wei T
病毒和宿主细胞都竞争细胞内多胺以有效繁殖。目前，关键的多胺代谢酶，包括鸟氨酸脱羧酶1（ODC 1）和其抗酶1（OAZ 1），如何被激活，以协调病毒繁殖和多胺生物合成仍然未知。在这里，我们报告了水稻条纹花叶病毒（RSMV），一种细胞弹状病毒的基质蛋白，直接劫持OAZ 1，以确保正确的组装刚性杆状无包膜病毒粒子在叶蝉载体。病毒基质蛋白有效地与ODC 1竞争结合OAZ 1，因此，在病毒繁殖期间，OAZ 1靶向和介导ODC 1降解的能力被显著抑制，这最终促进多胺产生。因此，OAZ 1和ODC 1被激活以协同促进病毒载体中的病毒持续繁殖和多胺生物合成。我们的数据表明，弹状病毒利用OAZ 1促进病毒组装是一种新的机制。在线版本包含补充材料，可通过10.1007/s44154-021-00032-z获得。
Both viruses and host cells compete for intracellular polyamines for efficient propagation. Currently, how the key polyamine-metabolizing enzymes, including ornithine decarboxylase 1 (ODC1) and its antizyme 1 (OAZ1), are activated to co-ordinate viral propagation and polyamine biosynthesis remains unknown. Here, we report that the matrix protein of rice stripe mosaic virus (RSMV), a cytorhabdovirus, directly hijacks OAZ1 to ensure the proper assembly of rigid bacilliform non-enveloped virions in leafhopper vector. Viral matrix protein effectively competes with ODC1 to bind to OAZ1, and thus, the ability of OAZ1 to target and mediate the degradation of ODC1 is significantly inhibited during viral propagation, which finally promotes polyamines production. Thus, OAZ1 and ODC1 are activated to synergistically promote viral persistent propagation and polyamine biosynthesis in viruliferous vectors. Our data suggest that it is a novel mechanism for rhabdovirus to exploit OAZ1 for facilitating viral assembly. The online version contains supplementary material available at 10.1007/s44154-021-00032-z.
影响因子：
3.7
作者：
Hsieh JY;Yang JY;Lin CL;Liu GY;Hung HC
通讯作者：
Hung HC