Method for determining the lung burden of talc in rats and mice after inhalation exposure to talc aerosols.
Method for determining the lung burden of talc in rats and mice after inhalation exposure to talc aerosols.
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大鼠和小鼠吸入暴露于滑石气溶胶后确定滑石粉肺负荷的方法。
DOI：
10.1002/jat.2550050504
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Brown,SC
中科院分区：
文献类型：
--
作者：
Hanson,RL;Benson,JM;Henderson,TR;Carpenter,RL;Pickrell,JA;Brown,SC
已经开发了一种方法来定量吸入暴露于滑石粉气溶胶后大鼠和小鼠中的滑石粉肺负荷。该方法基于火焰原子吸收法测定酸不溶性镁（Mg）。用5%高氯酸和5%三氯乙酸洗涤未暴露啮齿动物肺匀浆中的蛋白质，除去可溶性和天然存在的镁。这导致残留镁含量平均为0.43 μg镁/g肺的大鼠和小于12周龄的年轻啮齿动物的小鼠小于0.1 μg镁/g肺。12-18月龄啮齿动物的残留平均（±SD）镁含量为3.4 ± 2.0 μg Mg/g大鼠肺（n=17）和6.5 ± 2.9 μg Mg/g小鼠肺（n=12）。因此，啮齿类动物肺中的背景残留酸不溶性镁含量似乎随年龄增加而增加。可忽略不计的量的镁直接从这些程序处理的滑石中提取。将50-2000 μg滑石粉加入未暴露啮齿动物的肺中，然后进行样品处理，得出大鼠肺和小鼠肺的平均（±SD）Mg回收率分别为89 ± 12%（n=19）和96 ± 26%（n=15）。测定了暴露于滑石粉气雾剂的啮齿动物中滑石粉的肺负荷，每天6 h，每周5天，持续4周。在2.3、4.3和17 mg滑石粉m3暴露量下，大鼠的平均肺负荷分别为77、187和806 μg滑石粉/g肺（n=10）。对于2.2、6.3和20.6 mg滑石粉m3的暴露量，小鼠的平均肺负荷分别为114、325和1150 μg滑石粉/g肺（n=10）。不同接触组的肺负荷的变异系数在8%至21%之间。
A method has been developed to quantitate talc lung burdens in rats and mice after inhalation exposure to talc aerosols. The method is based on acid‐insoluble magnesium (Mg) determination by flame atomic absorption. Precipitating protein from homogenates of lungs of unexposed rodents with 5% perchloric acid and washing with 5% trichloroacetic acid removed the soluble and naturally occurring Mg. This resulted in residual Mg content averaging 0.43 μg Mg per g lung in rats and less than 0.1 μg Mg per g lung in mice for young rodents less than 12 weeks old. Rodents 12–18 months old had residual mean (±SD) Mg contents of 3.4 ± 2.0 μg Mg per g rat lung (n=17) and 6.5 ± 2.9 μg Mg per g mouse lung (n=12). Thus, the background residual acid‐insoluble Mg content in rodent lungs appears to increase with age. Negligible quantities of Mg were extracted directly from the talc treated by these procedures. Adding 50–2000 μg talc to lungs from unexposed rodents, followed by the sample treatment, gave mean (±SD) Mg recoveries of 89 ± 12% (n=19) for rat lungs and 96 ± 26% (n=15) for mouse lungs. The lung burden of talc in rodents exposed to talc aerosols for 6 h per day, 5 days per week for 4 weeks was determined. Mean lung burdens in rats were 77, 187, and 806 μg talc per g lung (n=10) for exposures at 2.3, 4.3, and 17 mg talc m3, respectively. Mean lung burdens in mice were 114, 325 and 1150 μg talc per g lung (n=10) for exposures at 2.2, 6.3 and 20.6 mg talc m3, respectively. The coefficient of variation for the lung burdens of the different exposure groups ranged from 8 to 21%.