Electrocautery-induced cavernous nerve injury in rats that mimics radical prostatectomy in humans
Electrocautery-induced cavernous nerve injury in rats that mimics radical prostatectomy in humans
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DOI：
10.1111/bju.12348
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发表时间：
2014-07-01
影响因子：
4.5
通讯作者：
Xu, Yue-Min
中科院分区：
文献类型：
--
作者：
Song, Lu-Jie;Zhu, Jian-Qiang;Xu, Yue-Min
目的探讨大鼠海绵体神经电灼损伤（CNEI）的早期和延迟效应，以期为根治性阴茎切除术（RP）后勃起功能障碍（艾德）的康复治疗提供实验依据。对照组仅行CNs暴露术，实验组行双侧CNEI，于术后1、4、16周（每个时间点5只）测定两组大鼠最大海绵体内压（ICP）与平均动脉压（MAP）的比值。在阴茎背神经中的神经丝表达通过免疫荧光染色和Masson三色染色进行评估，以评估平滑肌胶原蛋白的比例在两个group.ResultsAt 1周的后续行动，平均ICP/MAP是显着低于CNEI组与对照组相比，9.94% vs 70.06% CNEI组的平均ICP/MAP在4- 10时显著增加（P < 0.05）。（35.97%）和16周（37.11%）与1周随访相比CNEI组神经丝染色较对照组明显减少（P <0. 05）。CNEI组4周和16周时神经丝蛋白表达均显著高于1周时（P < 0.05），但4周与16周之间差异无统计学意义术后4周和16周，CNEI组的平滑肌/胶原比值明显低于对照组（P <0.05）16周时较4周时进一步降低（P < 0.05）结论在CNEI大鼠模型中，CNEI可引起颅内压下降，阴茎背神经纤维数目减少，这可能为研究潜在的预防措施或治疗策略以改善RP期间CNEI引起的艾德提供了基础。
ObjectiveTo investigate the early and delayed effects of cavernous nerve electrocautery injury (CNEI) in a rat model, with the expectation that this model could be used to test rehabilitation therapies for erectile dysfunction (ED) after radical prostatectomy (RP).Materials and MethodsIn all, 30 male Sprague-Dawley rats were randomly divided equally into two groups (15 per group). The control group received CNs exposure surgery only and the experimental group received bilateral CNEI.At 1, 4 and 16 weeks after surgery (five rats at each time point), the ratio of maximal intracavernosal pressure (ICP) to mean arterial pressure (MAP) was measured in the two groups. Neurofilament expression in the dorsal penile nerves was assessed by immunofluorescent staining and Masson's trichrome staining was used to assess the smooth muscle to collagen ratio in both groups.ResultsAt the 1-week follow-up, the mean ICP/MAP was significantly lower in the CNEI group compared with the control group, at 9.94% vs 70.06% (P < 0.05).The mean ICP/MAP in the CNEI group was substantially increased at the 4- (35.97%) and 16-week (37.11%) follow-ups compared with the 1-week follow-up (P < 0.05).At all three follow-up time points, the CNEI group had significantly decreased neurofilament staining compared with the control group (P < 0.05). Also, neurofilament expressions in the CNEI group at both 4 and 16 weeks were significantly higher than that at 1 week (P < 0.05), but there was no difference between 4 and 16 weeks (P > 0.05).The smooth muscle to collagen ratio in the CNEI group was significantly lower than in the control group at the 4- and 16-week follow-ups (P < 0.05), and the ratio at 16 weeks was further reduced compared with that at 4 weeks (P < 0.05).ConclusionsIn the CNEI rat model, we found the damaging effects of CNEI were accompanied by a decline in ICP, reduced numbers of nerve fibres in the dorsal penile nerve, and exacerbated fibrosis in the corpus cavernosum.This may provide a basis for studying potential preventative measures or treatment strategies to ameliorate ED caused by CNEI during RP.