In vivo corticotropin-releasing factor-induced secretion of adrenocorticotropin, beta-endorphin, and corticosterone.
In vivo corticotropin-releasing factor-induced secretion of adrenocorticotropin, beta-endorphin, and corticosterone.
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DOI：
10.1210/endo-110-1-272
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发表时间：
1982
期刊：
影响因子：
4.8
通讯作者：
C. Rivier;M. Brownstein;J. Spiess;J. Rivier;W. Vale
中科院分区：
文献类型：
--
作者：
C. Rivier;M. Brownstein;J. Spiess;J. Rivier;W. Vale
在三个动物组中测试体外纯化为促肾上腺皮质激素释放因子/β-内啡肽释放因子（CRF）的41个残基的肽刺激ACTH、β-内啡肽和皮质酮分泌的能力：1）未麻醉的大鼠，其带有留置的静脉插管，2）用氯丙嗪+硫酸吗啡+戊巴比妥预处理的大鼠（CPZ-MS-Nb）和3）在额叶和外侧视交叉后区具有下丘脑传入阻滞的大鼠。在所有三种生物测定中，静脉注射0.1-10微克CRF引起血浆ACTH和β-内啡肽值在5- 15分钟内呈剂量相关性增加。皮质酮分泌也升高，但最大限度地响应所有剂量的CRF测试。试验前4小时用20微克地塞米松预处理CPZ-MS-Nb动物，可消除CRF诱导的激素分泌。这些数据表明，CRF可能在下丘脑-垂体-肾上腺轴的调节中发挥生理作用。
A 41-residue peptide purified as a corticotropin-releasing factor/beta-endorphin-releasing factor (CRF) in vitro was tested for its ability to stimulate the secretion of ACTH, beta-endorphin, and corticosterone in three animal groups: 1) unanesthesized rats bearing indwelling venous cannulae, 2) rats pretreated with chloropromazine plus morphine sulfate plus pentobarbital (CPZ-MS-Nb, and 3) rats with hypothalamic deafferentiations in the frontal and lateral retrochiasmatic areas. In all three bioassays iv administration of 0.1-10 micrograms CRF elicited a dose-related increase in plasma ACTH and beta-endorphin values over a 5- to 15-min period. Corticosterone secretion was also elevated but responded maximally with all doses of CRF tested. Pretreatment of CPZ-MS-Nb animals with 20 micrograms dexamethasone 4 h before assay abolished the CRF-induced hormone secretion. These data suggest that CRF may play a physiological role in the regulation of the hypothalamic-pituitary-adrenal axis.