Separation of fluorescent oligosaccharide derivatives by microcolumn techniques based on electrophoresis and liquid chromatography.
Separation of fluorescent oligosaccharide derivatives by microcolumn techniques based on electrophoresis and liquid chromatography.
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基于电泳和液相色谱的微柱技术分离荧光寡糖衍生物。
DOI：
10.1016/0021-9673(91)80073-p
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Novotny,M
中科院分区：
文献类型：
--
作者：
Liu,JP;Shirota,O;Novotny,M
各种醛糖低聚糖可以定量衍生为伯胺，用于随后与荧光试剂如3-（4-羧基苯甲酰基）-2-喹啉甲醛或3-苯甲酰基-2-萘甲醛反应。毛细管电泳（CE）和微柱液相色谱（LC），再加上激光诱导荧光检测，作为一种手段，分离复杂的寡糖混合物进行了评估。而微柱LC和开放式管状CE出现局限于其实用程序相对较小的寡糖，前所未有的结果得到了聚丙烯酰胺凝胶填充的毛细管水解的麦芽寡糖和酶降解的硫酸软骨素和透明质酸的样品。
Various aldose oligosaccharides can be quantitatively derivatized into primary amines for subsequent reaction with fluorogenic reagents, such as 3-(4-carboxybenzoyl)-2-quinolinecarboxaldehyde or 3-benzoyl-2-naphthaldehyde. Capillary electrophoresis (CE) and microcolumn liquid chromatography (LC), coupled with laser-induced fluorescence detection, were evaluated as a means of separating complex oligosaccharide mixtures. Whereas microcolumn LC and open-tubular CE appear confined in their utility to relatively small oligosaccharides, unprecedented results were obtained with polyacrylamide gel-filled capillaries on hydrolysed malto-oligosaccharides and enzymatically degraded samples of chondroitin sulfate and hyaluronic acid.