CD8+ T cells recognize an inclusion membrane-associated protein from the vacuolar pathogen Chlamydia trachomatis
CD8+ T cells recognize an inclusion membrane-associated protein from the vacuolar pathogen Chlamydia trachomatis
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DOI：
10.1073/pnas.98.3.1160
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发表时间：
2001-01-30
影响因子：
11.1
通讯作者：
Starnbach, MN
中科院分区：
文献类型：
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作者：
Fling, SP;Sutherland, RA;Starnbach, MN
在沙眼衣原体感染过程中，CD8（+）T细胞被激活，即使细菌在整个发育周期中仍然局限于宿主细胞空泡中。由于CD8+ T细胞识别由胞质蛋白加工的抗原，因此这些CD8（+）T细胞识别的衣原体抗原很可能在感染期间进入宿主细胞的细胞质。这些C.沙眼蛋白仍然是难以捉摸的，即使它们的定位表明它们可能在有机体的生物学中起重要作用。在这里，我们使用逆转录病毒表达系统，以确定Cap1，一个31 kDa的蛋白质从C。保护性CD8（+）T细胞识别沙眼衣原体。Cap1与任何已知蛋白质都没有很强的同源性。通过使用Cap1特异性抗体的免疫荧光显微镜表明，该蛋白质定位于液泡膜。Cap1在人类C.这表明包含Cap1的疫苗可能使该疫苗能够保护免受所有沙眼衣原体血清型的侵害。鉴定与包涵体膜相关的蛋白质，如Cap1，将需要充分理解C.沙眼衣原体与其宿主细胞。
During infection with Chlamydia trachomatis, CD8(+) T cells are primed, even though the bacteria remain confined to a host cell vacuole throughout their developmental cycle. Because CD8+ T cells recognize antigens processed from cytosolic proteins, the Chlamydia antigens recognized by these CD8(+) T cells very likely have access to the host cell cytoplasm during infection. The identity of these C. trachomatis proteins has remained elusive, even though their localization suggests they may play important roles in the biology of the organism. Here we use a retroviral expression system to identify Cap1, a 31-kDa protein from C. trachomatis recognized by protective CD8(+) T cells. Cap1 contains no strong homology to any known protein. Immunofluorescence microscopy by using Cap1-specific antibody demonstrates that this protein is localized to the vacuolar membrane. Cap1 is virtually identical among the human C. trachomatis serovars, suggesting that a vaccine incorporating Cap1 might enable the vaccine to protect against all C.trachomatis serovars. The identification of proteins such as Cap1 that associate with the inclusion membrane will be required to fully understand the interaction of C. trachomatis with its host cell.