Expression of H-2 and Moloney leukemia virus-determined cell-surface antigens in synchronized cultures of a mouse cell line.
Expression of H-2 and Moloney leukemia virus-determined cell-surface antigens in synchronized cultures of a mouse cell line.
复制标题
H-2 和莫洛尼白血病病毒确定的细胞表面抗原在小鼠细胞系同步培养物中的表达。
DOI：
--
复制
发表时间：
1971
影响因子：
11.1
通讯作者：
S. Friberg
中科院分区：
文献类型：
--
作者：
M. Cikes;S. Friberg
用莫洛尼白血病病毒体外感染小鼠骨髓细胞系（JLS-V9）。在这种病毒特异性的细胞表面抗原出现后，用秋水仙胺短时间处理细胞使有丝分裂同步化。H-2和病毒确定的表面抗原的表达在一个细胞周期期间通过间接膜免疫荧光试验进行监测。在G1期发现抗原阳性细胞的比例最高;随着细胞进入S期，该比例下降，并保持低水平，直到它们进入下一个周期的G1期。H-2和病毒确定的表面抗原的时间共表达。
A mouse cell line derived from bone marrow (JLS-V9) was infected in vitro with Moloney leukemia virus. After the cell-surface antigens specified by this virus appeared, cells were synchronized in mitosis by a short treatment with colcemid. The expression of H-2- and virus-determined surface antigens was monitored during one cell cycle by an indirect membrane-immunofluorescence test. The highest proportion of antigen-positive cells was found during the G1 period; the proportion dropped as the cells entered the S period, and remained low until they entered the G1 period of the next cycle. The H-2- and virus-determined surface antigens were temporally coexpressed.