Establishment of a novel volume-loaded heterotopic heart transplantation model in rats.
Establishment of a novel volume-loaded heterotopic heart transplantation model in rats.
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新型大鼠体积负荷异位心脏移植模型的建立。
DOI：
10.1016/j.jss.2013.01.018
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Zhihai Peng
中科院分区：
文献类型：
--
作者：
Peihao Wen;Xiaoliang Wang;Tao Li;Bo Zhang;Xing Sun;G. Qiu;Junwei Fan;Tonghai Xing;Qiongzhen Luo;Hua;Zhihai Peng
大鼠异位心脏移植模型中的非容量负荷（NL）供体心脏在移植后经历移植物腔中的萎缩和血栓形成。本研究旨在建立一种新的容量负荷供心模型。将供体升主动脉和肺动脉分别与受体腹主动脉和下腔静脉端侧吻合，并在供体右心房上结扎上级和下腔静脉，建立NL模型。容量负荷（VL）模型的方法与上述相同，不同之处在于我们将供体左心房与受体腹主动脉吻合，以允许供体左心室的容量负荷。通过手术成功率、超声心动图和组织学评价评价两种模型移植物的特点，结果显示，与NL模型相比，VL模型供者左心室呈容积负荷，收缩和舒张功能正常。NL心脏的平均重量明显小于VL心脏。形态学观察显示，NL模型供心腔内血栓形成明显高于VL模型。NL模型每高倍视野的心肌细胞面积明显低于VL模型。结论我们提供了一种新的大鼠VL异位心脏移植模型，移植物的血流动力学性能接近正常心脏生理状态，因此，该模型将更适合于临床研究。
BACKGROUNDNon–volume-loaded (NL) donor hearts in the heterotopic heart transplantation model in rats undergo atrophy and thrombus formation in graft cavities after transplantation. The present study aimed to establish a novel model with volume-loaded donor hearts.METHODSWe used Sprague-Dawley rats as donors and recipients. We established an NL model by anastomosing the donor ascending aorta and pulmonary artery end-to-side to the recipient abdominal aorta and inferior vena cava, respectively, and ligating the superior and inferior vena cava on the donor right atrium. The method of the volume-loaded (VL) model was the same as described above, except we performed an anastomosis of the donor left atrium to the recipient abdominal aorta to allow volume loading of the donor's left ventricle. We assessed the characteristics of the grafts by the surgical success rate, echocardiography, and histologic evaluation between the two models.RESULTSEchocardiography showed that donor left ventricle in VL models was volume loaded and had normal systolic and diastolic function compared with the NL models. The mean weight of NL hearts was significantly less than that of VL hearts. Morphologic observation revealed that thrombus formation in donor heart cavities in NL model was significantly higher than that in the VL model. The area of cardiomyocytes per high-power field in the NL model was significantly lower than that in the VL model.CONCLUSIONSWe provide a novel VL heterotopic heart transplantation model in rats, in which hemodynamic performance of grafts is close to the normal cardiac physiologic situation; thus, the novel model will be more suitable for clinical research.
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非容量负荷心脏提供了更相关的异位移植模型。
影响因子：
1.5
作者：
Tang-Quan,KarisR;Bartos,Jason;Deuse,Tobias;Churchill,Eric;Schäfer,Hansjörg;Reichenspurner,Hermann;Mochly-Rosen,Daria;Robbins,RobertC;Schrepfer,Sonja
通讯作者：
Schrepfer,Sonja
使用通过磁共振成像评估的工作心脏模型评估移植大鼠心脏的心脏功能。
DOI：
10.1016/s1053-2498(99)00077-7
发表时间：
1999
期刊：
The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation
影响因子：
--
作者：
Yoshida,S;Dodd,SJ;delNido,PJ;Williams,DS;Ho,C
通讯作者：
Ho,C
异位移植大鼠心脏的心肌萎缩：体外蛋白质合成。
影响因子：
5
作者：
Klein,I;Hong,C;Schreiber,SS
通讯作者：
Schreiber,SS
影响因子：
2.8
作者：
Booth AJ;Bishop DK
通讯作者：
Bishop DK