CHROMagar™ Candida Plus: A novel chromogenic agar that permits the rapid identification of Candida auris
CHROMagar™ Candida Plus: A novel chromogenic agar that permits the rapid identification of Candida auris
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DOI：
10.1093/mmy/myaa049
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发表时间：
2021-03-01
期刊：
影响因子：
2.9
通讯作者：
Johnson, Elizabeth M.
中科院分区：
文献类型：
--
作者：
Borman, Andrew M.;Fraser, Mark;Johnson, Elizabeth M.
耳念珠菌是一种严重的医院健康风险，在世界各地的医院中广泛爆发。成功地控制这种疾病爆发取决于对病人进行密集的筛查，以确定哪些人是定植者，并随后对受影响的病人进行隔离或集中，以防止进一步传播。在这里，我们描述了一种新的显色琼脂，CHROMagar（TM）念珠菌加，从患者样本中的耳念珠菌分离株的特异性鉴定的评价。在CHROMagar（TM）Candida Plus上的耳念珠菌菌落为淡奶油色，具有扩散到周围琼脂中的独特蓝色晕圈。在平行培养的50多个不同种类的念珠菌和相关属中，只有消失的罕见种类迪登念珠菌给出了类似的外观。此外，无论是生长速度和菌落数的C。与在传统真菌学分离培养基沙氏葡萄糖琼脂上的生长相比，在CHROMagarTM CandidaPlus琼脂上，从纯的和混合的念珠菌属物种的拭子中回收的耳内念珠菌的生长显著增加。综上所述，目前的数据表明，CHROMagar（TM）Candida Plus琼脂是目前常规真菌培养基的极好替代品，用于筛选可能被耳念珠菌定殖/感染的患者，可以可靠地用于鉴定这种新出现的真菌病原体，摘要耳念珠菌是一种新的致病性酵母菌，与大规模的医院暴发有关横跨几个大洲。受影响的病人成为殖民地，主要是在皮肤上，与大量的C。然后他们把它们撒到医院环境中。C.鉴定使用常规实验室方法检测耳内念珠菌感染具有挑战性，并且当患者被不同念珠菌属的混合物定殖时耗时。在这里，我们证明了一种新的显色琼脂，CHROMagar（TM）念珠菌加，允许快速分化的C。耳中的酵母菌，并且可能理想地适用于筛选怀疑被这种医学上重要的酵母菌定殖或感染的患者。
Candida auris is a serious nosocomial health risk, with widespread outbreaks in hospitals worldwide. Successful management of such outbreaks has depended upon intensive screening of patients to identify those that are colonized and the subsequent isolation or cohorting of affected patients to prevent onward transmission. Here we describe the evaluation of a novel chromogenic agar, CHROMagar (TM) Candida Plus, for the specific identification of Candida auris isolates from patient samples. Candida auris colonies on CHROMagar (TM) Candida Plus are pale cream with a distinctive blue halo that diffuses into the surrounding agar. Of over 50 different species of Candida and related genera that were cultured in parallel, only the vanishingly rare species Candida diddensiae gave a similar appearance. Moreover, both the rate of growth and number of colonies of C. auris recovered from swabs of pure and mixed Candida species were substantially increased on CHROMagar (TM) Candida Plus agar when compared with growth on the traditional mycological isolation medium, Sabouraud dextrose agar. Taken together, the present data suggest that CHROMagar (TM) Candida Plus agar is an excellent alternative to current conventional mycological media for the screening of patients who are potentially colonized/infected with Candida auris, can be reliably used to identify this emerging fungal pathogen, and should be tested in a clinical setting.Lay AbstractCandida auris is a novel pathogenic yeast that has been associated with large hospital outbreaks across several continents. Affected patients become colonized, predominantly on the skin, with large quantities of C. auris which they then shed into the hospital environment. Identification of C. auris is challenging using routine laboratory methods, and time consuming when patients are colonized with a mixture of different Candida species. Here we demonstrate that a novel chromogenic agar, CHROMagar (TM) Candida Plus, permits the rapid differentiation of C. auris from a wide range of other yeast species and is potentially ideally suited to screening of patients that are suspected of being colonized or infected with this medically important yeast.