Adherence of Streptococcus pneumoniae to polystyrene plates, effect of serum on adhesion, and virulence in the gerbil otitis media model
Adherence of Streptococcus pneumoniae to polystyrene plates, effect of serum on adhesion, and virulence in the gerbil otitis media model
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DOI：
10.1016/j.micpath.2007.05.005
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发表时间：
2007-08-01
影响因子：
3.8
通讯作者：
Soriano, Francisco
中科院分区：
文献类型：
--
作者：
Huelves, Lorena;del Prado, Gema;Soriano, Francisco
研究了11株肺炎链球菌对聚苯乙烯的黏附情况，并以每10⁶个初始接种菌落形成单位（CFU）中回收的CFU数量来表示。3株被视为强黏附（>100 CFU/10⁶），3株为中等黏附（10 - 100 CFU/10⁶），5株为低黏附（<10 CFU/10⁶）。所有血清型3的菌株均为低黏附，而血清型23F和19F表现为强黏附或中等黏附。还在两种实验条件下研究了沙鼠血清对6株选定的肺炎链球菌菌株（1株强黏附、1株中等黏附和4株低黏附）对非生物材料黏附的影响。在有血清存在的情况下，强黏附、中等黏附和1株低黏附菌株的黏附能力显著下降。另一方面，当细菌在接触血清预培养后去除血清时，所有菌株的黏附都显著增加，尽管其中5株的这种增加具有统计学意义。最后，还评估了2株（1株强黏附和1株低黏附）菌株在沙鼠中诱发中耳炎的能力；就耳部损伤和动物体重减轻而言，强黏附菌株的毒性明显高于黏附性较弱的菌株。（c）2007爱思唯尔有限公司。保留所有权利。
The adherence of 11 pneumococcal strains to polystyrene was studied and expressed as the number of colony-forming units (CFU) recovered per 10(6) CFU of initial inoculum. Three strains were considered as strong adherent (> 100 CFU/10(6)), three as medium adherent (10-100 CFU/10(6)), and five as low adherent (< 10 CFU/10(6)). All serotype 3 strains were low adherent whilst serotypes 23F and 19F behaved as strong or medium adherent. The impact of gerbil sera on adherence of six selected pneumococcal strains (one strong adherent, one medium adherent, and four low adherents) to abiotic material was also studied under two experimental conditions. In the presence of sera, the adherence ability of the strong, medium, and one low adherent strains decreased significantly. On the other hand, the adherence significantly increased in all strains when sera were removed following preincubation of bacteria exposed to sera, although such increase was statistically significant for five of them. Finally, the ability of two (one strong adherent and one low adherent) strains to induce otitis media in gerbils was also evaluated; the strong adherent strain behaved significantly more virulent than the less adherent in terms of ear damage and animal weight loss. (c) 2007 Elsevier Ltd. All rights reserved.