Assessment of cross-reactivity between Japanese cedar (Cryptomeria japonica) pollen and tomato fruit extracts by RAST inhibition and immunoblot inhibition
Assessment of cross-reactivity between Japanese cedar (Cryptomeria japonica) pollen and tomato fruit extracts by RAST inhibition and immunoblot inhibition
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DOI：
10.1046/j.0954-7894.2002.01337.x
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发表时间：
2002-04-01
影响因子：
6.1
通讯作者：
Matsuda, T
中科院分区：
文献类型：
--
作者：
Kondo, Y;Tokuda, R;Matsuda, T
背景花粉症和对水果和蔬菜的敏感性之间的联系已经被报道。虽然日本雪松日本柳杉花粉症是日本最普遍的疾病之一，日本雪松花粉与其他植物性食品之间的交叉反应性尚未有报道。目的本研究旨在证明日本雪松花粉与番茄果实之间的交叉反应性（Lycopersicon esculentum）方法采用RAST抑制法和免疫印迹抑制法对口腔变态反应综合征患者血清进行RAST和免疫印迹抑制法检测（OAS）后ingergly新鲜西红柿。通过N-末端氨基酸序列测定和SWISS-PROT数据库同源性检索，确定了部分参与交叉反应IgE的蛋白质。通过将血清与番茄果实提取物预孵育，来自五个受试者中的四个（4/5）受试者的血清的IgE与日本雪松花粉圆片的结合被抑制超过50%。在315份血清中，日本雪松花粉提取物对番茄果盘的IgE结合抑制超过50%。在免疫印迹抑制中，两种膜上的一些蛋白条带的IgE结合活性被异源抑制剂降低。然而，这些蛋白质条带的组合涉及交叉反应性patients.Conclusion，我们已经证明了日本雪松花粉和番茄果实之间的交叉反应性RAST抑制和免疫印迹抑制。
Background An association between pollinosis and sensitivity to fruits and vegetables has been reported. Although Japanese cedar (Cryptomeria japonica) pollinosis is one of the most widespread diseases in Japan, there have been no reports demonstrating cross-reactivity between Japanese cedar pollen and other plant food.Objective The aim of this study was to demonstrate cross-reactivity between Japanese cedar pollen and tomato fruit (Lycopersicon esculentum) using RAST inhibition and immunoblot inhibition.Methods The RAST and immunoblot inhibition were performed using sera from patients with oral allergy syndrome (OAS) after ingesting fresh tomatoes. We identified some proteins that took part in cross-reactive IgE by the determination of N-terminal animo acid sequences and a homology search through the SWISS-PROT database.Results In the RAST inhibition. the bindings of IgE from the sera from four out of five (4/5) subjects to Japanese cedar pollen discs were inhibited by more than 50% by preincubation of the serum with tomato fruit extracts, Likewise. the IgE bindings to tomato fruit discs were inhibited more than 50% by Japanese cedar pollen extracts in 315 sera. In immunoblot inhibition, IgE binding activities of some protein bands on both membranes were decreased by heterologous inhibitors. However, the combinations of these protein bands involved in cross-reactivity were different between patients.Conclusion We have demonstrated cross-reactivity between Japanese cedar pollen and tomato fruit using RAST inhibition and immunoblot inhibition.