CHOLINERGIC NEURONS IN THE BRAIN OF A TELEOST FISH (PORICHTHYS-NOTATUS) LOCATED WITH A MONOCLONAL-ANTIBODY TO CHOLINE-ACETYLTRANSFERASE
CHOLINERGIC NEURONS IN THE BRAIN OF A TELEOST FISH (PORICHTHYS-NOTATUS) LOCATED WITH A MONOCLONAL-ANTIBODY TO CHOLINE-ACETYLTRANSFERASE
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DOI：
10.1002/cne.902750108
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发表时间：
1988-09-01
影响因子：
2.5
通讯作者：
BASS, AH
中科院分区：
文献类型：
--
作者：
BRANTLEY, RK;BASS, AH
利用胆碱乙酰转移酶（ChAT）单克隆抗体（Ab8）在硬骨鱼midshipman （Porichthys notatus）脑中定位具有ChAT样免疫反应性（ChAT- ir）的结构。ChAT是乙酰胆碱的合成酶，存在于使用这种神经递质的神经元中；因此，ChAT-IR可以解释为表明假定的胆碱能活性。所有脑神经运动核均可见明显的染色。此外，波鱼的脑干由另外两个扩张的ChAT-IR区区分开来：声速运动核，支配膀胱“鼓”肌；八侧神经传出核，支配声学、前庭和侧线末端器官。在几个颅感觉核、迷走神经叶、三叉神经主核和下行核中发现了分散的标记细胞。ChAT-IR细胞在其他非颅神经核中形成有限的亚群，包括小脑的颗粒细胞层；网状结构的上、中、下分区；中脑视神经顶盖脑室周围层；中脑被盖中的峡核。在端脑，腹侧区腹侧核内有大量的ChAT-IR细胞；背区核的背核、内侧核和中央核中有终末和细纤维。总之，这些数据代表了具有Ab8单克隆抗体的非哺乳动物大脑中ChAT-IR细胞体的第一个完整报告，该单克隆抗体已广泛用于各种动物大脑。因此，本文从比较的角度讨论了不同分类群中有关ChAT-IR的报道。
A monoclonal antibody (Ab8) to choline acetyltransferase (ChAT) was used to locate structures showing ChAT-like immunoreactivity (ChAT-IR) in the brain of a teleost fish, the midshipman (Porichthys notatus). ChAT is the synthetic enzyme for acetylcholine found in neurons using that neurotransmitter; thus ChAT-IR may be interpreted as indicating putative cholinergic activity. Robust staining is seen in all cranial nerve motor nuclei. In addition, the brainstem of Porichthys is distinguished by two other expansive ChAT-IR zones: a sonic motor nucleus, which innervates swimbladder "drum" muscles, and an octavolateralis efferent nucleus, which innervates acoustic, vestibular, and lateral line end organs. Scattered labeled cells are found in several cranial sensory nuclei-the vagal lobe, and the main and descending trigeminal nuclei. ChAT-IR cells form restricted subpopulations in other noncranial nerve nuclei, including the granule cell layer of the cerebellum; superior, medial, and inferior divisions of the reticular formation; the stratum periventriculare of the midbrain''s optic tectum; and the nucleus isthmi in the midbrain tegmentum. In the telencephalon, a dense population of ChAT-IR cells is found in the ventral nucleus of area ventralis; terminals and fine fibers are found in the dorsal, medial, and central nuclei of area dorsalis. Together, the data represent the first complete report of ChAT-IR cell bodies in the brain of any nonmammal with the monoclonal antibody Ab8, which has already been extensively used on a variety of verebrate brains. The results are thus discussed from a comparative viewpoint, considering reports of ChAT-IR in different taxa.