Ovine placental lactogen stimulates glycogen synthesis in fetal rat hepatocytes.
Ovine placental lactogen stimulates glycogen synthesis in fetal rat hepatocytes.
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羊胎盘催乳素刺激胎鼠肝细胞中的糖原合成。
DOI：
10.1152/ajpendo.1984.246.1.e21
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Handwerger,S
中科院分区：
文献类型：
--
作者：
Freemark,M;Handwerger,S
在20日龄胎鼠肝细胞中研究了胎盘乳原对糖原代谢的影响。绵羊胎盘乳原（oPL； 2、5、10和25微克/毫升）在孵育4小时后刺激了[14C]葡萄糖入糖原和细胞总糖原含量的剂量依赖性增加，但对糖原降解没有影响。当oPL浓度为3微克/毫升时，出现半最大刺激。相比之下，人胎盘乳原对[14C]葡萄糖并入糖原没有影响。羊生长激素（50和100微克/毫升）、大鼠生长激素（20、40和100微克/毫升）和羊催乳素（10、40和100微克/毫升）刺激[14C]葡萄糖并入糖原的剂量依赖性增加，但这些激素的效力仅为oPL的10-20%。胰岛素（20 nM）刺激[14C]葡萄糖并入糖原，而胰高血糖素（0.5 nM和20 nM）抑制[14C]葡萄糖并入糖原并增加糖原降解。我们的研究结果表明，oPL可能对胎儿的碳水化合物代谢有直接的胰岛素样影响，并且oPL可能有助于妊娠后期胎儿肝糖原的积累。
The effects of placental lactogen on glycogen metabolism have been studied in cultured hepatocytes from 20-day-old fetal rats. Ovine placental lactogen (oPL; 2, 5, 10, and 25 micrograms/ml) stimulated dose-dependent increases in [14C]glucose incorporation into glycogen and total cellular glycogen content after 4 h of incubation but had no effect on glycogen degradation. Half-maximal stimulation occurred with an oPL concentration of 3 micrograms/ml. In contrast, human placental lactogen had no effect on [14C]glucose incorporation into glycogen. Ovine growth hormone (50 and 100 micrograms/ml), rat growth hormone (20, 40, and 100 micrograms/ml), and ovine prolactin (10, 40, and 100 micrograms/ml) stimulated dose-dependent increases in [14C]glucose incorporation into glycogen, but the potencies of these hormones were only 10-20% of that of oPL. Insulin (20 nM) stimulated [14C]glucose incorporation into glycogen, whereas glucagon (0.5 and 20 nM) inhibited [14C]glucose incorporation into glycogen and increased glycogen degradation. Our findings suggest that oPL may have direct insulin-like effects on carbohydrate metabolism in the fetus and that oPL may contribute to the accumulation of fetal liver glycogen that occurs in late gestation.