ARTHROPATHIC PROPERTIES RELATED TO THE MOLECULAR-WEIGHT OF PEPTIDOGLYCAN-POLYSACCHARIDE POLYMERS OF STREPTOCOCCAL CELL-WALLS
ARTHROPATHIC PROPERTIES RELATED TO THE MOLECULAR-WEIGHT OF PEPTIDOGLYCAN-POLYSACCHARIDE POLYMERS OF STREPTOCOCCAL CELL-WALLS
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DOI：
10.1128/iai.35.3.1003-1010.1982
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发表时间：
1982-01-01
影响因子：
3.1
通讯作者：
SCHWAB, JH
中科院分区：
文献类型：
--
作者：
FOX, A;BROWN, RR;SCHWAB, JH
从A群链球菌细胞壁中分离肽聚糖和群特异性多糖的共价结合聚合物（PG-APS）。通过单次腹腔注射超声处理产生的PG-APS片段的水悬浮液，在大鼠中诱导关节炎。用这种多分散性混悬液诱导的关节病变遵循由急性期组成的双峰模式，急性期在注射后5天达到高峰，然后消退，随后是持续数个月的慢性、缓解性、糜烂性关节炎。急性期和慢性期的相对严重程度可以通过选择PG-APS碎片的大小来控制。通过蔗糖梯度离心或差速离心，将超声处理获得的PG-APS片段根据大小分为3个主要群体。基于光散射和凝胶过滤，最大片段家族的平均MW估计为约500 × 104。106，中间片段为50 × 106。106道尔顿;最小群体中的主要大小为5.3 × 106道尔顿。106道尔顿。较大的碎片引起的急性炎症可以忽略不计，但慢性疾病在注射后5-9周变得明显。最小的碎片引起最严重的急性炎症，相对较少的晚期慢性关节疾病。中等大小的颗粒诱导中度急性炎症和最严重的慢性侵蚀性关节病变。单次注射PG-APS的分离的肽聚糖部分的片段仅诱导关节的中度急性炎症，没有明显的维持损伤和诱导慢性疾病的能力。
The covalently bound polymers of peptidoglycan and group-specific polysaccharide (PG-APS) were isolated from the cell walls of group A streptococci. Arthritis was induced in rats with a singe i.p. injection of an aqueous suspension of PG-APS fragments derived by sonication. The joint lesions induced with this polydisperse suspension followed a bimodal pattern consisting of an acute phase, which reached a peak 5 days after injection and then receded, followed by a chronic, remittent, erosive arthritis lasting several mo. The relative severities of the acute and chronic phases could be manipulated by selection of the size of PG-APS fragments. The fragments of PG-APS obtained by sonic treatment were resolved on the basis of size into 3 major populations by sucrose gradient or differential centrifugation. Based upon light scattering and gel filtration, the average MW of the largest family of fragments was estimated to be about 500 .times. 106 and the intermediate fragments were 50 .times. 106 daltons; the predominant size in the smallest population was 5.3 .times. 106 daltons. The larger fragments induced negligible acute inflammation, but chronic disease became apparent 5-9 wk after injection. The smallest fragments induced the most severe acute inflammation, with relatively little late, chronic joint disease. The particles of intermediate size induced moderate acute inflammation and the most severe chronic, erosive joint lesions. A single injection of fragments of the isolated peptidoglycan moiety of the PG-APS induced only a moderate acute inflammation of joints, with no apparent capacity to maintain the injury and induce chronic disease.