Interleukin-17 mRNA expression in blood and CSF mononuclear cells is augmented in multiple sclerosis
Interleukin-17 mRNA expression in blood and CSF mononuclear cells is augmented in multiple sclerosis
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DOI：
10.1191/135245899678847275
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发表时间：
1999-04-01
影响因子：
5.8
通讯作者：
Link, H
中科院分区：
文献类型：
--
作者：
Matusevicius, D;Kivisäkk, P;Link, H
髓磷脂定向的自身免疫被认为在多发性硬化症（MS）的发病机制中发挥着关键作用。促炎细胞因子和抗炎细胞因子的产生增加是多发性硬化症的常见发现。白细胞介素-17 (IL-17) 是最近描述的一种在人体中几乎完全由活化的记忆 T 细胞产生的细胞因子，它诱导实质细胞和巨噬细胞产生促炎细胞因子和趋化因子。采用合成寡核苷酸探针原位杂交来检测和计数 MS 患者和对照个体的血液和脑脊液 (CSF) 中表达 IL-17 mRNA 的单核细胞 (MNC)。与健康个体相比，MS 和急性无菌性脑膜脑炎 (AM) 患者血液中表达 IL-17 mRNA 的 MNC 数量更高。与缓解期相比，在临床恶化期间检查的 MS 患者中检测到更高数量的表达 IL-17 mRNA 的血液 MNC。与血液相比，多发性硬化症患者的脑脊液中表达 IL-17 mRNA 的 MNC 数量更高。 AM 患者中未观察到脑脊液中表达 IL-17 mRNA 的 MNC 数量增加。因此，我们的结果表明，MS 中表达 IL-17 mRNA 的 MNC 数量增加，其中脑脊液中的数量高于血液中的数量，并且在临床恶化期间血液中的数量最高。
Myelin-directed autoimmunity is considered to Ploy a key role in the pathogenesis of multiple sclerosis (MS). increased Production of both pro- and anti-inflammatory cytokines is a common finding in MS. Interleukin-17 (IL-17) is a recently described cytokine produced in humans almost exclusively by activated memory T cells, which con induce the production of proinflammatory cytokines and chemokines from parenchymal cells and macrophages. In situ hybridisation with synthetic oligonucleotide probes was adopted to detect and enumerate IL-17 mRNA expressing mononuclear cells (MNC) in blood and cerebrospinal fluid (CSF) from patients with MS and control individuals. Numbers of IL-17 mRNA expressing blood MNC were higher in patients with MS and acute aseptic meningoencephalitis (AM) compared to healthy individuals. Higher numbers of IL-17 mRNA expressing blood MNC were detected in MS patients examined during clinical exacerbation compared to remission. Patients with MS had higher numbers of IL-17 mRNA expressing MNC in CSF compared to blood. This increase in numbers of IL-17 mRNA expressing MNC in CSF was not observed in patients with AM. Our results thus demonstrate increased numbers of IL-17 mRNA expressing MNC in MS with higher numbers in CSF than blood, and with the highest numbers in blood during clinical exacerbations.