Larvicidal activity of Bacillus thuringiensis natural isolates, indigenous to Japan, against two nematoceran insect pests occurring in urban sewage environments

Larvicidal activity of Bacillus thuringiensis natural isolates, indigenous to Japan, against two nematoceran insect pests occurring in urban sewage environments
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DOI:
10.1016/s0944-5013(96)80023-1
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发表时间:
1996-08-01
影响因子:
6.7
通讯作者:
Ohba, M
Ohba, M
中科院分区:
生物学2区
文献类型:
--
作者:
Saitoh, H;Higuchi, K;Ohba, M

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对日本本土的共1449株苏云金芽孢杆菌菌株进行了针对两种长角亚目害虫——骚扰库蚊(蚊科)和白星蛾蚋(蛾蚋科)的杀幼虫活性筛选。在H血清型3abc(库斯塔克亚种)、3ade(福冈亚种)、4ac(肯尼亚亚种)、7( aizawai亚种)、11ac(九州亚种)和29(天城亚种)中,对蚊子有特异性的菌株数量丰富,而对蛾蚋有特异性的菌株主要在H血清型17(东北亚种)中发现。对两种昆虫都有毒性的菌株在H血清型10(达姆斯塔特亚种)和17/27中最为常见。7株选定的苏云金芽孢杆菌菌株对库蚊和/或白星蛾蚋有高毒性。这些菌株的包含体蛋白的SDS - PAGE图谱存在多样性。
A total of 1449 Bacillus thuringiensis strains, indigenous to Japan, were screened for larvicidal activity against two nematoceran insect pests, the mosquito, Culex pipiens molestus (Culicidae), and the moth-fly, Telmatoscopus albipunctatus (Psychodidae). Mosquito specific strains were abundant in H serotypes 3abc (serovar kurstaki), 3ade (fukuokaensis), 4ac (kenyae), 7 (aizawai), 11ac (kyushuensis) and 29 (amagiensis), while moth-fly specific strains were predominantly found in H serotype 17 (tohokuensis). Strains toxic to both insects were most frequently detected in H serotypes 10 (darmstadiensis) and 17/27. Seven selected B. thuringiensis strains were highly toxic to Culex and/or Telmatoscopus. There was a diversity in SDS-PAGE profiles of inclusion proteins of these strains.