Electrochemical Quantification of Glycated and Non-glycated Human Serum Albumin in Synthetic Urine
Electrochemical Quantification of Glycated and Non-glycated Human Serum Albumin in Synthetic Urine
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DOI：
10.1021/acsami.8b16071
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发表时间：
2019-02-06
影响因子：
9.5
通讯作者：
Weiss, Gregory A.
中科院分区：
文献类型：
--
作者：
Attar, Aisha M.;Richardson, Mark B.;Weiss, Gregory A.
本发明描述了一种能够特异性检测人血清白蛋白及其糖化衍生物的聚合物基电极。该传感器是由一个玻璃显微镜载玻片涂有合成，聚噻吩膜轴承保护，亚氨基二乙酸基序。然后通过亚氨基二乙酸的脱保护，然后通过特异性和高亲和力的白蛋白配体的金属亲和固定，将电极表面进一步加工成功能性生物传感器。然后通过电化学阻抗谱在缓冲液和合成尿液中定量白蛋白。糖化白蛋白接下来结合到硼酸修饰的单半胱氨酸二氢叶酸还原酶变体，以通过方波伏安法定量糖化率。该平台以廉价的方式提供高灵敏度、特异性和再现性。白蛋白及其糖化形式的检测限分别为5和1 nM，超过了糖尿病患者中期血糖控制监测的要求。
A polymer-based electrode capable of specific detection of human serum albumin, and its glycated derivatives, is described. The sensor is constructed from a glass microscope slide coated with a synthesized, polythiophene film bearing a protected, iminodiacetic acid motif. The electrode surface is then further elaborated to a functional biosensor through deprotection of the iminodiacetic acid, followed by metal-affinity immobilization of a specific and high-affinity, albumin ligand. Albumin was then quantified in buffer and synthetic urine via electrochemical impedance spectroscopy. Glycated albumin was next bound to a boronic acid-modified, single-cysteine dihydrofolate reductase variant to quantify glycation ratios by square wave voltammetry. The platform offers high sensitivity, specificity, and reproducibility in an inexpensive arrangement. The detection limits exceed the requirements for intermediate-term glycemic control monitoring in diabetes patients at 5 and 1 nM for albumin and its glycated forms, respectively.