TISSUE ABLATION BY A FREE-ELECTRON LASER TUNED TO THE AMIDE-II BAND
TISSUE ABLATION BY A FREE-ELECTRON LASER TUNED TO THE AMIDE-II BAND
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DOI：
10.1038/371416a0
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发表时间：
1994-09-29
期刊：
影响因子：
64.8
通讯作者：
ODAY, D
中科院分区：
文献类型：
--
作者：
EDWARDS, G;LOGAN, R;ODAY, D
使用传统激光消融软组织的努力受到附带损伤和对潜在光化学效应的担忧的限制(1-5)。受热约束模型 (6) 的推动，过去的红外研究以水的 OH 拉伸模式为目标，使用发射近 3,000 nm 的激光发出的快速脉冲（参考文献 1、7-9）。自由电子激光器为组织消融研究提供了什么？这些红外源在非光化学单光子能量下工作，可以产生一系列皮秒脉冲，可调节蛋白质、脂质和/或水的振动模式。我们在此报告，将自由电子激光辐射瞄准蛋白质的酰胺 II 带会导致组织消融，其特点是附带损伤最小，同时保持较高的消融率。为了解释这些观察结果，我们提出了一种基于通过结构蛋白共振变性损害组织的新型消融机制。
EFFORTS to ablate soft tissue with conventional lasers have been limited by collateral damage and by concern over potential photochemical effects(1-5). Motivated by the thermal-confinement model(6), past infrared investigations targeted the OH-stretch mode of water with fast pulses from lasers emitting near 3,000 nm (refs 1, 7-9). What does a free-electron laser offer for the investigation of tissue ablation? Operating at non-photochemical single-photon energies, these infrared sources can produce trains of picosecond pulses tunable to the vibrational modes of proteins, lipids and/or water. We report here that targeting free-electron laser radiation to the amide II band of proteins leads to tissue ablation characterized by minimal collateral damage while maintaining a substantial ablation rate. To account for these observations we propose a novel ablation mechanism based on compromising tissue through resonant denaturation of structural proteins.