Cathelicidin anti-microbial peptide expression in sweat, an innate defense system for the skin
Cathelicidin anti-microbial peptide expression in sweat, an innate defense system for the skin
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DOI：
10.1046/j.1523-1747.2002.19507.x
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发表时间：
2002-11-01
影响因子：
6.5
通讯作者：
Gallo, RL
中科院分区：
文献类型：
--
作者：
Murakami, M;Ohtake, T;Gallo, RL
小汗腺是皮肤的主要附属物之一，分泌汗液。它的主要功能是在暴露于热环境或体育锻炼期间进行体温调节。除了这个功能，我们表明，LL-37，抗菌肽cathelicidin家族的成员，在汗液中表达。LL-37蛋白和mRNA在正常人皮肤的外分泌结构中的免疫组织化学和原位杂交。LL-37定位于外分泌腺和汗腺导管上皮细胞，而dermcidin，一种先前描述的汗液中的天然抗生素，仅在腺体本身中表达。LL-37和杀皮素对汗液离子环境中的各种细菌的抗微生物活性通过使用合成肽的溶液菌落形成测定和在从正常志愿者获得的汗液中证明。这些结果表明，凯萨林菌素在人汗液中分泌，对革兰氏阳性和革兰氏阴性细菌都具有有效的抗微生物活性，并且在从预成型体加工后，可以提供保护免受感染的屏障。因此，汗液代表了在没有炎症的情况下有效的先天免疫效应分子的独特递送模式。
The eccrine gland is one of the major cutaneous appendages and secretes sweat. Its principal function is thermoregulation during exposure to a hot environment or physical exercise. In addition to this function, we show that LL-37, a member of cathelicidin family of antimicrobial peptides, is expressed in sweat. LL-37 protein and mRNA was seen in the eccrine structures of normal human skin by immunohistochemistry and in situ hybridization. LL-37 was localized to both the eccrine gland and sweat ductal epithelial cells, whereas dermcidin, a previously described natural antibiotic in sweat, was expressed only in the gland itself. The anti-microbial activity of LL-37 and dermcidin against various bacteria in the sweat ionic environment was demonstrated by solution colony forming assay using synthetic peptides, and in sweat obtained from normal volunteers. These results indicate that cathelicidin is secreted in human sweat, has potent anti-microbial activity against both gram-positive and gram-negative bacteria, and can, after processing from the preproform, provide a barrier for protection against infection. Thus, sweat represents a unique mode of delivery for potent innate immune effector molecules in the absence of inflammation.