Investigation of destruxin A and B from 80 Metarhizium strains in China, and the optimization of cultural conditions for the strain MaQ10
Investigation of destruxin A and B from 80 Metarhizium strains in China, and the optimization of cultural conditions for the strain MaQ10
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DOI：
10.1016/j.toxicon.2006.06.018
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发表时间：
2006-10-01
期刊：
影响因子：
2.8
通讯作者：
Musa, Peter D.
中科院分区：
文献类型：
--
作者：
Hu, Qiong-Bo;Ren, Shun-Xiang;Musa, Peter D.
研究了80株绿僵菌（Metarhizium）的腐败毒素A和B（DA和DB）的产生水平。DA和DB的平均产量分别为14.87 ± 2.30和3.65 ± 0.58 μ g/mL。从土壤中分离的菌株的DA和DB产量显著低于从昆虫尸体中分离的菌株（p < 0.05）。DA和DB的产量呈正相关，并建立了回归方程。在80株菌株中，MaQ 10菌株的DA和DB产量最高，在10天发酵期分别达到106.78 ± 9.41和29.52 ± 2.63 μ g/mL。其次是菌株MaQ 05、MaQ 07和MaQ 12，它们的DA产量分别为79.72 ± 5.36、77.69 ± 8.54和70.04 ± 10.1 μ g/mL，DB分别为16.81 ± 1.51、13.38 ± 0.41和16.88 ± 1.15 μ g/mL。此外，还对MaQ 10的培养条件进行了优化，以生产DA和DB。最佳接种量、初始pH、温度和转速分别为：DA为8%、pH 9.0、27 ℃和240 r/min; DB为8%、pH 9.0、25 ℃和220 r/min。在此最佳培养条件下，DA和DB的预测产量分别为193.87和39.85 μ g/mL。实际产量分别为189.13和43.35 μ g/mL。(c)2006爱思唯尔有限公司保留所有权利。
The production levels of destruxin A and B (DA and DB) of 80 Metarhizium strains from China were investigated. The average yields of DA and DB were 14.87 +/- 2.30 and 3.65 +/- 0.58 mu g/mL, respectively. The strains isolated from soil had significantly (p < 0.05) lower production of DA and DB than the strains isolated from insect cadavers. Also, there was a positive correlation in the yields of DA and DB, and the regressive equations were established. Of the 80 strains, MaQ10 had the highest yields of DA and DB, amounted to 106.78 +/- 9.41 and 29.52 +/- 2.63 mu g/mL, respectively, at 10 days fermentation period. Followed by the strains MaQ05, MaQ07 and MaQ12, their yields of DA were 79.72 +/- 5.36, 77.69 +/- 8.54 and 70.04 +/- 10.1 mu g/mL, respectively, and DB were 16.81 +/- 1.51, 13.38 +/- 0.41 and 16.88 +/- 1.15 mu g/mL, respectively. Furthermore, the cultural conditions of MaQ10 were optimized to produce DA and DB. The optimal inoculum, initial pH, temperature and rotary speed were 8%, pH 9.0,27 degrees C and 240 r/min for DA, and 8%, pH 9.0,25 degrees C and 220 r/min for DB. Under these optimal culture conditions, the predicted production of DA and DB was 193.87 and 39.85 mu g/mL.. and the actual production was 189.13 and 43.35 mu g/mL, respectively. (c) 2006 Elsevier Ltd. All rights reserved.