Induction of Acute Skeletal Muscle Regeneration by Cardiotoxin Injection
Induction of Acute Skeletal Muscle Regeneration by Cardiotoxin Injection
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DOI：
10.3791/54515
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发表时间：
2017-01-01
影响因子：
1.2
通讯作者：
Minchiotti, Gabriella
中科院分区：
文献类型：
--
作者：
Guardiola, Ombretta;Andolfi, Gennaro;Minchiotti, Gabriella
骨骼肌再生是一个生理过程，发生在成年骨骼肌中，以应对损伤或疾病。急性损伤诱导的骨骼肌再生是一种广泛使用的，功能强大的模型系统，用于研究肌肉再生中涉及的事件以及机制和不同的参与者。事实上，详细了解这一过程对于更好地理解导致骨骼肌变性的病理条件至关重要，并且有助于确定新的靶向治疗策略。本工作描述了一个详细的和可重复的协议，以诱导急性骨骼肌再生小鼠通过一个单一的肌肉注射心脏毒素（CTX）。CTX属于蛇毒毒素家族，并导致肌纤维的肌溶解，最终触发再生事件。通过肌肉切片的组织学分析来评估骨骼肌再生的动力学。该方案还说明了解剖、冷冻和切割胫骨前肌的实验程序，以及广泛用于后续形态学和形态测定分析的常规苏木精和伊红染色。
Skeletal muscle regeneration is a physiological process that occurs in adult skeletal muscles in response to injury or disease. Acute injury-induced skeletal muscle regeneration is a widely used, powerful model system to study the events involved in muscle regeneration as well as the mechanisms and different players. Indeed, a detailed knowledge of this process is essential for a better understanding of the pathological conditions that lead to skeletal muscle degeneration, and it aids in identifying new targeted therapeutic strategies. The present work describes a detailed and reproducible protocol to induce acute skeletal muscle regeneration in mice through a single intramuscular injection of cardiotoxin (CTX). CTX belongs to the family of snake venom toxins and causes myolysis of myofibers, which eventually triggers the regeneration events. The dynamics of skeletal muscle regeneration is evaluated by histological analysis of muscle sections. The protocol also illustrates the experimental procedures for dissecting, freezing, and cutting the Tibialis Anterior muscle, as well as the routine Hematoxylin & Eosin staining that is widely used for subsequent morphological and morphometric analysis.